Document Detail

Hemoglobin is expressed by mesangial cells and reduces oxidant stress.
MedLine Citation:
PMID:  18448584     Owner:  NLM     Status:  MEDLINE    
Hemoglobin (Hb) serves as the main oxygen transporter in erythrocytes, but it is also expressed in nonhematopoietic organs, where it serves an unknown function. In this study, microarray and proteomic analyses demonstrated Hb expression in the kidney. Rat kidneys were perfused extensively with saline, and glomeruli were isolated by several techniques (sieving, manual dissection, and laser capture-microdissection). Reverse transcriptase-PCR revealed glomerular alpha- and beta-globin expression, and immunoblotting demonstrated expression of the protein. In situ hybridization studies showed expression of the globin subunits in the mesangium, and immunostaining confirmed this localization of Hb. Furthermore, globin mRNA expression was detected in primary cultures of rat mesangial cells but not in cultured glomerular endothelial or epithelial cells. For investigation of Hb function in mesangial cells, the SV40-MES13 murine mesangial cell line was transfected with a vector expressing alpha- and beta-globins; this overexpression reduced production of hydrogen peroxide-induced intracellular radical oxygen species and enhanced cell viability against oxidative stress. In summary, Hb is expressed by rat mesangial cells, and its potential functions may include antioxidative defense.
Hiroshi Nishi; Reiko Inagi; Hideki Kato; Masayuki Tanemoto; Ichiro Kojima; Daisuke Son; Toshiro Fujita; Masaomi Nangaku
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-30
Journal Detail:
Title:  Journal of the American Society of Nephrology : JASN     Volume:  19     ISSN:  1533-3450     ISO Abbreviation:  J. Am. Soc. Nephrol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-29     Completed Date:  2008-08-20     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9013836     Medline TA:  J Am Soc Nephrol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1500-8     Citation Subset:  IM    
Division of Nephrology and Endocrinology, University of Tokyo School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.
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MeSH Terms
Anoxia / metabolism*
Cells, Cultured
Fluorescent Antibody Technique
Gene Expression
Hemoglobins / genetics,  metabolism*
In Situ Hybridization
Mesangial Cells / metabolism*
Oxidative Stress*
RNA, Messenger / metabolism
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Hemoglobins; 0/RNA, Messenger
Comment In:
J Am Soc Nephrol. 2008 Aug;19(8):1440-1   [PMID:  18614770 ]

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