| Hemodynamics is a key epigenetic factor in development of the cardiac conduction system. | |
| | |
MedLine Citation:
|
PMID: 12775585 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The His-Purkinje system (HPS) is a network of conduction cells responsible for coordinating the contraction of the ventricles. Earlier studies using bipolar electrodes indicated that the functional maturation of the HPS in the chick embryo is marked by a topological shift in the sequence of activation of the ventricle. Namely, at around the completion of septation, an immature base-to-apex sequence of ventricular activation was reported to convert to the apex-to-base pattern characteristic of the mature heart. Previously, we have proposed that hemodynamics and/or mechanical conditioning may be key epigenetic factors in development of the HPS. We thus hypothesized that the timing of the topological shift marking maturation of the conduction system is sensitive to variation in hemodynamic load. Spatiotemporal patterns of ventricular activation (as revealed by high-speed imaging of fluorescent voltage-sensitive dye) were mapped in chick hearts over normal development, and following procedures previously characterized as causing increased (conotruncal banding, CTB) or reduced (left atrial ligation, LAL) hemodynamic loading of the embryonic heart. The results revealed that the timing of the shift to mature activation displays striking plasticity. CTB led to precocious emergence of mature HPS function relative to controls whereas LAL was associated with delayed conversion to apical initiation. The results from our study indicate a critical role for biophysical factors in differentiation of specialized cardiac tissues and provide the basis of a new model for studies of the molecular mechanisms involved in induction and patterning of the HPS in vivo. |
| | |
Authors:
|
Maria Reckova; Carlin Rosengarten; Angela deAlmeida; Chiffvon P Stanley; Andy Wessels; Robert G Gourdie; Robert P Thompson; David Sedmera |
Related Documents
:
|
9237415 - Recurrent branch retinal infarcts in association with migraine. 2816145 - The conduction system in dilated cardiomyopathy. a pathomorphological study. 12411095 - Complications of percutaneous transluminal septal myocardial ablation in hypertrophic o... 1137655 - Acute inferior wall myocardial infarction associated with complete atrioventricular blo... 10700065 - Balloon positioning difficulties during nonsurgical septal reduction therapy in a patie... 17437575 - Elimination of fluoroscopy use in a pediatric electrophysiology laboratory utilizing th... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. Date: 2003-05-29 |
Journal Detail:
|
Title: Circulation research Volume: 93 ISSN: 1524-4571 ISO Abbreviation: Circ. Res. Publication Date: 2003 Jul |
Date Detail:
|
Created Date: 2003-07-11 Completed Date: 2003-09-26 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0047103 Medline TA: Circ Res Country: United States |
Other Details:
|
Languages: eng Pagination: 77-85 Citation Subset: IM |
Affiliation:
|
Department of Cell Biology and Anatomy, Medical University of South Carolina, 173 Ashley Ave, BSB 601, Charleston, SC 29425, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chick Embryo Heart Conduction System / embryology, physiology*, physiopathology Heart Ventricles / embryology, physiopathology Hemodynamics Hypoplastic Left Heart Syndrome / embryology, metabolism, physiopathology Immunohistochemistry Myocardium / chemistry Neural Cell Adhesion Molecule L1 / analysis Purkinje Fibers / embryology, physiology*, physiopathology Sialic Acids / analysis Ventricular Function |
| Grant Support | |
ID/Acronym/Agency:
|
HD39946/HD/NICHD NIH HHS; HL50582/HL/NHLBI NIH HHS; HL52813/HL/NHLBI NIH HHS; RR16434/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Neural Cell Adhesion Molecule L1; 0/Sialic Acids; 0/polysialyl neural cell adhesion molecule |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: EphA4-mediated Rho activation via Vsm-RhoGEF expressed specifically in vascular smooth muscle cells.
Next Document: Role of the cytosolic chaperones Hsp70 and Hsp90 in maturation of the cardiac potassium channel HERG...