Document Detail

Hemodynamic responses and c-Fos changes associated with hypotensive hemorrhage: standardizing a protocol for severe hemorrhage in conscious rats.
MedLine Citation:
PMID:  17218446     Owner:  NLM     Status:  MEDLINE    
The central mechanisms underlying the transition from compensation to decompensation during severe hemorrhage (HEM) are poorly understood. Furthermore, a lack of consistency in HEM protocols exists in the current literature. This study assessed the cardiovascular response and Fos-like immunoreactivity (FLI) in specific brain regions following severe HEM at three rates (2, 1, or 0.5 in conscious rats. Heart rate (HR) and arterial pressure were recorded during the withdrawal of 30% of total blood volume (TBV). Data from animals hemorrhaged at the fast (F-HEM, n = 6), intermediate (I-HEM, n = 7), or slow (S-HEM, n = 7) rates were compared with saline (SAL, n = 5) and hypotensive (hydrazaline-induced, HYDRAZ, n = 5) controls. All HEM rates produced similar degrees of hypotension at the time of 30% TBV withdrawal. All HEM rates also produced bradycardia, but the change in HR was only significant in the F-HEM and I-HEM groups. Associated with I-HEM and F-HEM, but not HYDRAZ treatment were significant increases in FLI in the caudal ventrolateral periaqueductal gray (PAG), the central lateral nucleus of the rostral parabrachial nucleus, and locus coeruleus compared with SAL treatment. I-HEM also induced significant increases in FLI in the dorsomedial PAG, A7 region, and the cuneiform nucleus compared with SAL. S-HEM did not induce any significant change in FLI. Our results suggest that HEM at a rate of 1 may be most useful for investigating the potential role of the rostral brainstem regions in mediating hemorrhagic decompensation in conscious rats.
Joslyn Ahlgren; Karen Porter; Linda F Hayward
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-01-11
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  292     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-03     Completed Date:  2007-07-02     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R1862-71     Citation Subset:  IM    
Dept of Physiological Sciences, HSC, Univ of Florida, Gainesville, FL 32610, USA.
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MeSH Terms
Blood Pressure / drug effects,  physiology*
Blood Volume / drug effects,  physiology
Brain / metabolism
Heart Rate / drug effects,  physiology*
Hemorrhage / complications*,  metabolism*
Hydralazine / therapeutic use
Hypotension / complications*,  drug therapy,  physiopathology
Proto-Oncogene Proteins c-fos / metabolism*
Rats, Sprague-Dawley
Sodium Chloride / therapeutic use
Time Factors
Vasodilator Agents / therapeutic use
Grant Support
Reg. No./Substance:
0/Proto-Oncogene Proteins c-fos; 0/Vasodilator Agents; 7647-14-5/Sodium Chloride; 86-54-4/Hydralazine

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