Document Detail


Hemodynamic Contribution of Stem Cell Scaffolding in Acute Injured Myocardium.
MedLine Citation:
PMID:  22607369     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Tissue engineered scaffolds may improve experimental outcomes in cardiac cell therapy by targeted delivery of stem cells and mechanically support infarcted left ventricular wall. We transplanted cardiomyocyte-like cells (5 x 10<sup>5</sup>) with scaffolding via epicardial patching (cell patch, n=17) or low dose intra-myocardial hydrogel (LD hydrogel, n=18), high dose (5 x 10<sup>6</sup>) intra-myocardial hydrogel (HD hydrogel, n=18) or transplanting serum-free medium control (control, n=13), blank patch (n=14) and blank gel (n=16) for targeted cardiomyoplasty in a myocardial infarcted rat model. Left ventricular (LV) real-time hemodynamics were assessed using 1.9-F pressure-volume catheter 7 weeks after stem cell transplantation. All mode of scaffold transplantation protected diastolic function by preserving LV wall integrity that resulted in a lower end diastolic pressure volume relationship (EDPVR) as compared to control medium injected group. Moreover, epicardial patching, but not hydrogel injection, reduced ventricular wall stress with a significantly better LV end diastolic pressure (EDP: 5.3 ± 2.4 mmHg vs. 9.6 ± 6.9 mmHg, p<0.05) as compared to control. Furthermore, epicardial patching additionally preserved systolic function by modulating negative remodeling through restricting dilatation of LV chamber. In comparison to control, improved ejection fraction in the cell patch group (80.1 ± 5.9% vs. 67.9 ± 3.2%, p<0.01) was corroborated by load-independent enhancement of end systolic pressure volume relationship (ESPVR: 0.88 ± 0.61 mmHg/uL vs. 0.29 ± 0.19 mmHg/uL, p<0.05) and pre-load recruitable stroke work (PRSW: 68.7 ± 26.4 mmHg vs. 15.6 ± 16.2 mmHg, p<0.05) in systolic function. Moreover, cell patch group (14.2 ± 1.7 cells/high power field vs. 7.4 ± 1.6 cells/high power field, p<0.05) was significantly better in myocardial retention of transplanted stem cells as compared to LD hydrogel group. Collectively, myocardial transplantation of compliant scaffolding materials alone may physically improve wall mechanics, largely independent of stem cells. However, epicardially grafted cell patch conferred added systolic contractility by improving stem cell retention and cellular alignment leading to improved LV remodeling and geometric preservation post-infarction.
Authors:
Ling Qian; Winston Shim; Yacui Gu; Mohamed Shirhan; Kee Pah Lim; Lay Poh Tan; Chong Hee Lim; Yoong Kong Sin; Philip Wong
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-18
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Singapore, Singapore; daye24@gmail.com.
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