Document Detail


Hemocytes from the tobacco hornworm Manduca sexta have distinct functions in phagocytosis of foreign particles and self dead cells.
MedLine Citation:
PMID:  16054213     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phagocytosis is an important innate immune response against microbial infections and an effective mechanism to eliminate apoptotic cells. In vertebrates, phagocytes such as macrophages and dendritic cells are involved in phagocytosis. We demonstrate here that insect hemocytes have distinct functions in phagocytosis of foreign particles and self dead cells. Plasmatocytes from the tobacco hornworm Manduca sexta were major hemocytes involved in phagocytosis of non-self microsphere beads, whereas granulocytes were apparently the only hemocytes that phagocytose self dead cells. We also showed that M. sexta immulectin-2, a pattern recognition receptor that protects larvae from bacterial infection, has an opsonic activity in phagocytosis. Immulectin-2 bound to the surface of granulocytes from the naïve larvae, but more immulectin-2 bound to plasmatocytes when larvae were injected with microsphere beads. Coupling of immulectin-2 onto microsphere beads enhanced in vitro phagocytosis of the beads. Our results suggest that insect hemocytes can have specialized functions similar to vertebrate phagocytes in phagocytosis, and pattern recognition receptors may function as opsonins to enhance phagocytosis.
Authors:
Erjun Ling; Xiao-Qiang Yu
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Publication Detail:
Type:  Journal Article     Date:  2005-06-17
Journal Detail:
Title:  Developmental and comparative immunology     Volume:  30     ISSN:  0145-305X     ISO Abbreviation:  Dev. Comp. Immunol.     Publication Date:  2006  
Date Detail:
Created Date:  2005-11-07     Completed Date:  2006-04-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7708205     Medline TA:  Dev Comp Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  301-9     Citation Subset:  IM    
Affiliation:
Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autophagy / physiology*
Cell Death
Granulocytes / cytology,  physiology
Hemocytes / cytology*,  drug effects,  immunology,  physiology*
Infection
Insect Proteins / pharmacology
Lectins, C-Type
Manduca / cytology*,  drug effects,  physiology*
Microspheres
Phagocytosis / drug effects,  immunology*
Plasmacytoma
Chemical
Reg. No./Substance:
0/Insect Proteins; 0/Lectins, C-Type; 0/immulectin-2 protein, Manduca sexta

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