| Hemocompatibility of titania nanotube arrays. | |
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MedLine Citation:
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PMID: 20629021 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hemocompatibility is a key consideration for the long-term success of blood contacting biomaterials; hence, there is a critical need to understand the physiological response elicited from blood/nano-biomaterial interactions. In this study, we have investigated the adsorption of key blood serum proteins, in vitro adhesion and activation of platelets, and clotting kinetics of whole blood on titania nanotube arrays. Previous studies have demonstrated improved mesenchymal stem cell functionality, osteoblast phenotypic behavior, localized drug delivery, and the production of endothelial cell ECM on titania nanotube arrays. Furthermore, these titania nanotube arrays have elicited minimal levels of monocyte activation and cytokine secretion, thus exhibiting a very low degree of immunogenicity. Titania nanotube arrays were fabricated using anodization technique and the surface morphology was examined through scanning electron microscopy (SEM). The crystalline phases were identified using glancing angled X-ray diffraction (GAXRD). Nanoindentation and scratch tests were used to characterize the mechanical properties of titania nanotube arrays. The adsorption of key blood proteins (albumin, fibrinogen, and immunoglobulin-g) was evaluated using a micro-BCA assay and X-ray photoelectron spectroscopy (XPS). The adhesion and activation of platelets was investigated using live-cell staining, MTT assay, and SEM. Whole blood clotting kinetics was evaluated by measuring the free hemoglobin concentration, and SEM was used to visualize the clot formation. Our results indicate increased blood serum protein adsorption, platelet adhesion and activation, and whole blood clotting kinetics on titania nanotube arrays. |
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Authors:
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Barbara S Smith; Sorachon Yoriya; Laura Grissom; Craig A Grimes; Ketul C Popat |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 95 ISSN: 1552-4965 ISO Abbreviation: J Biomed Mater Res A Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-09-22 Completed Date: 2011-01-13 Revised Date: 2011-02-14 |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 350-60 Citation Subset: IM |
Affiliation:
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School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado 80523, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Biocompatible Materials / chemistry* Blood Proteins / chemistry Humans Materials Testing Nanotubes / chemistry* Platelet Adhesiveness Surface Properties Titanium / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Blood Proteins; 13463-67-7/titanium dioxide; 7440-32-6/Titanium |
| Comments/Corrections | |
Erratum In:
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J Biomed Mater Res A. 2011 Mar 1;96(3):608 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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