| Hemocompatibility assessment of poly (2-dimethylamino ethylmethacrylate) (PDMAEMA)-based polymers. | |
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MedLine Citation:
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PMID: 21550368 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Poly(2-dimethylamino-ethylmethacrylate) (PDMAEMA), a cationic polymer, has been widely reported as a nonviral carrier. Despite the fact that the cytotoxicity of this polymer has been extensively studied, there is a lack of information about its blood compatibility. Hence, this work evaluates the hemocompatibility of free-form PDMAEMA homopolymers differing in molecular weight (Mw) with or without a poly(ethylene glycol) (PEG) sequence in the form of a palm tree-like structure. Poly(ethylenimine) (PEI) was used as a reference in order to compare its hemoreactivity. Hemagglutination, hemolysis, platelet number, blood coagulation, and the complement systems were assessed in normal human whole blood according to the ISO 10993-4. Results showed that Mw, concentration, and incubation time strongly affected the hemocompatibility of the polymers evaluated. Our in vitro observations highlight that PDMAEMA homopolymers interacted strongly with the surface of the red blood cells but not with the inner structure of the membrane, while PEI behaved in the opposite way. No clear correlation has been evidenced between PDMAEMA-induced hemagglutination, PEI-induced hemagglutination, and hemolysis. Interestingly, if these polyelectrolytes strongly affect the platelets and blood coagulation cascades in a dose dependent way, none of them significantly affects the complement system. Our work reveals new knowledge on the toxicology of 2 families of polycations largely explored for gene delivery and on their mechanisms of cellular and humoral interactions. |
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Authors:
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Bernardino Isaac Cerda-Cristerna; Héctor Flores; Amaury Pozos-Guillén; Elias Perez; Chantal Sevrin; Christian Grandfils |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-28 |
Journal Detail:
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Title: Journal of controlled release : official journal of the Controlled Release Society Volume: - ISSN: 1873-4995 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-5-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8607908 Medline TA: J Control Release Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Interfacultary Research Center of Biomaterials, University of Liège, Institute of Chemistry, Building B6C, Sart-Tillman (Liège), Liège (ZIP code 4000), Belgium; Laboratory of Basic Sciences, University of San Luis Potosí, Faculty of Dentistry, Avenida Dr. Manuel Nava No. 2, San Luis Potosí, (ZIP code 78290), San Luis Potosí, México. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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