| Hemochromatosis and pregnancy: iron stores in the Hfe-/- mouse are not reduced by multiple pregnancies. | |
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MedLine Citation:
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PMID: 20110460 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hereditary hemochromatosis (HH), a widespread hereditary iron metabolism disorder, is characterized by an excessive absorption of dietary iron, resulting in increased body iron stores. Some studies indicate a sex difference in disease expression, with women showing a slower disease progression and a less severe clinical profile. This is usually attributed to iron loss during menstruation and pregnancy. However, this link has not been clearly demonstrated. The Hfe-/- mouse model recapitulates key aspects of HH, including an iron overload phenotype similar to that observed in human patients. In this study, we use it to test the impact of multiple pregnancies in the iron stores. One-year-old nulliparous and pluriparous (averaging 29 weaned pups per female) C57BL/6 (B6) and Hfe-/- mice were euthanized, and blood and tissues were collected. Several serological and erythroid parameters were evaluated, as well as tissue nonheme iron content and serum ferritin. Hepcidin 1, hepcidin 2, and bone morphogenetic protein 6 (BMP6) expressions in the liver were determined by real-time PCR. No significant differences were observed for many serological and erythroid parameters although differences occurred in transferrin saturation and mean corpuscular volume in Hfe-/- mice and total iron-binding capacity in B6 mice. Hepatic iron concentration was similar for nulliparous and pluriparous mice of both genotypes, but total iron per organ (liver, spleen, heart, and pancreas) was higher overall in pluriparous females than nulliparous. Hepcidin 1 and 2 and BMP6 expressions were significantly decreased in pluriparous females, when compared with nulliparous, in both genotypes. In conclusion, multiple pregnancies do not reduce body iron stores in Hfe-/- mice. |
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Authors:
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Jo?o Vilares Neves; Ingrid Anna Sofia Olsson; Gra?a Porto; Pedro Nuno Rodrigues |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-28 |
Journal Detail:
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Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 298 ISSN: 1522-1547 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-22 Completed Date: 2010-04-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: United States |
Other Details:
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Languages: eng Pagination: G525-9 Citation Subset: IM |
Affiliation:
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Iron Genes and Immune System (IRIS), Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antimicrobial Cationic Peptides / genetics Apoferritins / blood Bone Morphogenetic Protein 6 / genetics Erythrocyte Count Erythrocyte Indices / genetics Female Gene Expression / genetics Hematocrit Hemochromatosis / blood, complications*, genetics, metabolism* Hemoglobins / analysis, metabolism Histocompatibility Antigens Class I / genetics* Iron / analysis, blood, metabolism* Liver / chemistry, metabolism Membrane Proteins / genetics* Mice Mice, Inbred C57BL Mice, Knockout Myocardium / chemistry, metabolism Pancreas / chemistry, metabolism Parity / physiology* Pregnancy Pregnancy Complications / blood, genetics, metabolism* Spleen / chemistry, metabolism Transferrin / chemistry, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antimicrobial Cationic Peptides; 0/Bmp6 protein, mouse; 0/Bone Morphogenetic Protein 6; 0/Hamp2 protein, mouse; 0/Hemoglobins; 0/Hfe protein, mouse; 0/Histocompatibility Antigens Class I; 0/Membrane Proteins; 0/hepcidin; 11096-37-0/Transferrin; 7439-89-6/Iron; 9013-31-4/Apoferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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