Document Detail

Hemochromatosis and pregnancy: iron stores in the Hfe-/- mouse are not reduced by multiple pregnancies.
MedLine Citation:
PMID:  20110460     Owner:  NLM     Status:  MEDLINE    
Hereditary hemochromatosis (HH), a widespread hereditary iron metabolism disorder, is characterized by an excessive absorption of dietary iron, resulting in increased body iron stores. Some studies indicate a sex difference in disease expression, with women showing a slower disease progression and a less severe clinical profile. This is usually attributed to iron loss during menstruation and pregnancy. However, this link has not been clearly demonstrated. The Hfe-/- mouse model recapitulates key aspects of HH, including an iron overload phenotype similar to that observed in human patients. In this study, we use it to test the impact of multiple pregnancies in the iron stores. One-year-old nulliparous and pluriparous (averaging 29 weaned pups per female) C57BL/6 (B6) and Hfe-/- mice were euthanized, and blood and tissues were collected. Several serological and erythroid parameters were evaluated, as well as tissue nonheme iron content and serum ferritin. Hepcidin 1, hepcidin 2, and bone morphogenetic protein 6 (BMP6) expressions in the liver were determined by real-time PCR. No significant differences were observed for many serological and erythroid parameters although differences occurred in transferrin saturation and mean corpuscular volume in Hfe-/- mice and total iron-binding capacity in B6 mice. Hepatic iron concentration was similar for nulliparous and pluriparous mice of both genotypes, but total iron per organ (liver, spleen, heart, and pancreas) was higher overall in pluriparous females than nulliparous. Hepcidin 1 and 2 and BMP6 expressions were significantly decreased in pluriparous females, when compared with nulliparous, in both genotypes. In conclusion, multiple pregnancies do not reduce body iron stores in Hfe-/- mice.
Jo?o Vilares Neves; Ingrid Anna Sofia Olsson; Gra?a Porto; Pedro Nuno Rodrigues
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-28
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  298     ISSN:  1522-1547     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-22     Completed Date:  2010-04-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G525-9     Citation Subset:  IM    
Iron Genes and Immune System (IRIS), Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.
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MeSH Terms
Antimicrobial Cationic Peptides / genetics
Apoferritins / blood
Bone Morphogenetic Protein 6 / genetics
Erythrocyte Count
Erythrocyte Indices / genetics
Gene Expression / genetics
Hemochromatosis / blood,  complications*,  genetics,  metabolism*
Hemoglobins / analysis,  metabolism
Histocompatibility Antigens Class I / genetics*
Iron / analysis,  blood,  metabolism*
Liver / chemistry,  metabolism
Membrane Proteins / genetics*
Mice, Inbred C57BL
Mice, Knockout
Myocardium / chemistry,  metabolism
Pancreas / chemistry,  metabolism
Parity / physiology*
Pregnancy Complications / blood,  genetics,  metabolism*
Spleen / chemistry,  metabolism
Transferrin / chemistry,  metabolism
Reg. No./Substance:
0/Antimicrobial Cationic Peptides; 0/Bmp6 protein, mouse; 0/Bone Morphogenetic Protein 6; 0/Hamp2 protein, mouse; 0/Hemoglobins; 0/Hfe protein, mouse; 0/Histocompatibility Antigens Class I; 0/Membrane Proteins; 0/hepcidin; 11096-37-0/Transferrin; 7439-89-6/Iron; 9013-31-4/Apoferritins

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