Document Detail

Hemoccult stool tests: false-negative results due to storage of specimens.
MedLine Citation:
PMID:  957789     Owner:  NLM     Status:  MEDLINE    
Stoll samples mixed with 0 to 38 mg of hemoglobin per gram of stool were tested for occult blood by the Hemoccult (HO) method when fresh and after storage for 1 to 14 days. The intensity of the HO reaction in fresh stool samples was directly related to the hemoglobin concentration, and approximately 8 mg/g was required for a positive reaction. After samples had been stored on HO cards for 4 days, the intensity of the HO reaction was decidedly less in all. Except at the highest concentrations studied, all became HO-negative or trace within 8 days. In contrast, samples of most concentrations that were stored in tightly capped containers prior to preparation of HO cards maintained a positive reaction intensity for 1 week. Nevertheless, the intensity of the HO reaction decreased significantly (P less than 0.01) with each method of storage. No false-positives were induced by storage during this portion of our study. Samples from 100 stool specimens sent to the clinical laboratory for determination of occult blood were also smeared on separate HO cards for testing immediately and after 4, 7, or 10 days of storage. Only one positive HO reaction was observed on an HO card prepared from a specimen initially classified as HO-negative. In contrast, up to 40% of the stored HO cards prepared from specimens initially classified as HO-positive were HO-negative when analyzed 4 days later.
J R Stroehlein; V F Fairbanks; V L Go; W F Taylor; J H Thompson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Mayo Clinic proceedings     Volume:  51     ISSN:  0025-6196     ISO Abbreviation:  Mayo Clin. Proc.     Publication Date:  1976 Sep 
Date Detail:
Created Date:  1976-11-01     Completed Date:  1976-11-01     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0405543     Medline TA:  Mayo Clin Proc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  548-52     Citation Subset:  IM    
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MeSH Terms
False Negative Reactions
Feces / analysis
Guaiac / diagnostic use
Hemoglobins / analysis
Melena / diagnosis
Occult Blood*
Specimen Handling / methods*
Time Factors
Reg. No./Substance:
0/Hemoglobins; 9000-29-7/Guaiac

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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