| Hemin-induced suicidal erythrocyte death. | |
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MedLine Citation:
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PMID: 19184015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several diseases, such as malaria, sickle cell disease, and ischemia/reperfusion may cause excessive formation of hemin, which may in turn trigger hemolysis. A variety of drugs and diseases leading to hemolysis triggers suicidal erythrocyte death or eryptosis, i.e., cell membrane scrambling and cell shrinkage. Eryptosis is elicited by increased cytosolic Ca(2+) activity and by ceramide. The present study explored whether hemin stimulates eryptosis. Cell membrane scrambling was estimated from annexin V-binding to phosphatidylserine exposed at the cell surface, cell shrinkage from forward scatter in fluorescence-activated cell sorter analysis, cytosolic Ca(2+) activity from Fluo3 fluorescence and ceramide formation from fluorescence-labeled antibody binding. Exposure to hemin (1-10 microM) within 48 h significantly increased annexin V-binding, decreased forward scatter, increased cytosolic Ca(2+) activity, and stimulated ceramide formation. In conclusion, hemin stimulates suicidal cell death, which may in turn contribute to the clearance of circulating erythrocytes and thus to anemia. |
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Authors:
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Sergios Gatidis; Michael Föller; Florian Lang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-01-29 |
Journal Detail:
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Title: Annals of hematology Volume: 88 ISSN: 1432-0584 ISO Abbreviation: Ann. Hematol. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-06-17 Completed Date: 2009-07-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9107334 Medline TA: Ann Hematol Country: Germany |
Other Details:
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Languages: eng Pagination: 721-6 Citation Subset: IM |
Affiliation:
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Department of Physiology, University of Tübingen, Tübingen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Annexin A5
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metabolism Apoptosis / drug effects* Blood Cells / cytology Calcium / metabolism Cell Size Cells, Cultured Ceramides / biosynthesis Erythrocytes / cytology, drug effects* Hemin / pharmacology* Hemolysis / drug effects Humans Phosphatidylserines / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5; 0/Ceramides; 0/Phosphatidylserines; 16009-13-5/Hemin; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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