Document Detail


Hemin-induced suicidal erythrocyte death.
MedLine Citation:
PMID:  19184015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several diseases, such as malaria, sickle cell disease, and ischemia/reperfusion may cause excessive formation of hemin, which may in turn trigger hemolysis. A variety of drugs and diseases leading to hemolysis triggers suicidal erythrocyte death or eryptosis, i.e., cell membrane scrambling and cell shrinkage. Eryptosis is elicited by increased cytosolic Ca(2+) activity and by ceramide. The present study explored whether hemin stimulates eryptosis. Cell membrane scrambling was estimated from annexin V-binding to phosphatidylserine exposed at the cell surface, cell shrinkage from forward scatter in fluorescence-activated cell sorter analysis, cytosolic Ca(2+) activity from Fluo3 fluorescence and ceramide formation from fluorescence-labeled antibody binding. Exposure to hemin (1-10 microM) within 48 h significantly increased annexin V-binding, decreased forward scatter, increased cytosolic Ca(2+) activity, and stimulated ceramide formation. In conclusion, hemin stimulates suicidal cell death, which may in turn contribute to the clearance of circulating erythrocytes and thus to anemia.
Authors:
Sergios Gatidis; Michael Föller; Florian Lang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-29
Journal Detail:
Title:  Annals of hematology     Volume:  88     ISSN:  1432-0584     ISO Abbreviation:  Ann. Hematol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-06-17     Completed Date:  2009-07-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107334     Medline TA:  Ann Hematol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  721-6     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of Tübingen, Tübingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Annexin A5 / metabolism
Apoptosis / drug effects*
Blood Cells / cytology
Calcium / metabolism
Cell Size
Cells, Cultured
Ceramides / biosynthesis
Erythrocytes / cytology,  drug effects*
Hemin / pharmacology*
Hemolysis / drug effects
Humans
Phosphatidylserines / metabolism
Chemical
Reg. No./Substance:
0/Annexin A5; 0/Ceramides; 0/Phosphatidylserines; 16009-13-5/Hemin; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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