Document Detail

Hemin or butyrate increases constitutive erythropoietin formation by mouse erythroleukemia cell lines.
MedLine Citation:
PMID:  8330646     Owner:  NLM     Status:  MEDLINE    
The murine erythroleukemic cell lines NN10 and IW32 secrete erythropoietin (Epo) constitutively. Although both cell lines have Epo receptors (Epo-R), they do not respond to external Epo to undergo erythroid differentiation. In both cell lines, hemoglobin synthesis can be induced by hemin or butyrate. We report here on the effects of these two inducers on Epo production by the same cell lines. Both hemin and butyrate caused a three- to five-fold increase in Epo secretion on a percell basis. The effects were dose- and time-dependent, as was found previously for hemoglobin synthesis. The mechanism of the effect on Epo secretion is posttranscriptional, since the steady-state level of Epo mRNA was not altered by the inducers.
O Hermine; Y J Dong; N Beru; E Goldwasser
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental hematology     Volume:  21     ISSN:  0301-472X     ISO Abbreviation:  Exp. Hematol.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-08-19     Completed Date:  1993-08-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1207-11     Citation Subset:  IM    
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
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MeSH Terms
Butyrates / pharmacology*
Cell Count
Erythropoietin / genetics,  secretion*
Hemin / pharmacology*
Leukemia, Erythroblastic, Acute
RNA Processing, Post-Transcriptional
RNA, Messenger / analysis,  metabolism
Receptors, Erythropoietin / genetics
Time Factors
Tumor Cells, Cultured / metabolism*
Grant Support
Reg. No./Substance:
0/Butyrates; 0/RNA, Messenger; 0/Receptors, Erythropoietin; 11096-26-7/Erythropoietin; 16009-13-5/Hemin

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