| Hemeoxygenase-1 expression in response to arecoline-induced oxidative stress in human umbilical vein endothelial cells. | |
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MedLine Citation:
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PMID: 21889036 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: Arecoline, the most abundant areca alkaloid, has been reported to stimulate reactive oxygen species (ROS) production in several cell types. Overproduction of ROS has been implicated in atherogenesis. Hemeoxygenase-1 (HO-1) has cytoprotective activities in vascular tissues. This study investigated the effect of arecoline on adhesion molecule expression and explored the role of HO-1 in this process. METHODS: Human umbilical vein endothelial cells (HUVECs) were treated with arecoline, then ROS levels and the expression of adhesion molecules and HO-1 were analyzed and potential signaling pathways investigated. RESULTS: After 2h of arecoline treatment, ROS production was stimulated and reached a maximum at 12h. Expression of the adhesion molecules ICAM and VCAM was also induced. Glutathione pretreatment completely blocked arecoline-stimulated ROS production and VCAM expression, but not ICAM expression. Arecoline also induced HO-1 expression and this effect was partly due by ROS stimulation. Inhibition of c-jun N-terminal kinase (JNK) by SP600125, p38 by SB 203580, or tyrosine kinase by genistein reduced arecoline-induced HO-1 expression. In contrast, inhibition of ERK (extracellular signal-related MAP kinase) by PD98059 had no effect. Transfection of HUVECs with the GFP/HO-1 gene, which resulted in a 5-fold increase in HO-1 activity, markedly, but not completely, inhibited the decrease in cell viability caused by arecoline. CONCLUSIONS: This study demonstrates that, in HUVECs, arecoline stimulates ROS production and ICAM and VCAM expression. HO-1 expression is also upregulated through the ROS, tyrosine kinase, and MAPK (JNK and p38) signaling pathways. |
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Authors:
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Thu-Ching Hung; Li-Wen Huang; Shu-Jem Su; Bau-Shan Hsieh; Hsiao-Ling Cheng; Yu-Chen Hu; Yen-Hui Chen; Chi-Ching Hwang; Kee-Lung Chang |
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Publication Detail:
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Type: Journal Article Date: 2010-06-17 |
Journal Detail:
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Title: International journal of cardiology Volume: 151 ISSN: 1874-1754 ISO Abbreviation: Int. J. Cardiol. Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-09-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8200291 Medline TA: Int J Cardiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 187-94 Citation Subset: IM |
Copyright Information:
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Crown Copyright © 2010. Published by Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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