Document Detail


Haem oxygenase 1 expression is altered in monocytes from patients with systemic lupus erythematosus.
MedLine Citation:
PMID:  22587389     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by multiple functional alterations affecting immune cells, such as B cells, T cells, dendritic cells (DCs) and monocytes. During SLE, the immunogenicity of monocytes and DCs is significantly up-regulated, promoting the activation of self-reactive T cells. Accordingly, it is important to understand the contribution of these cells to the pathogenesis of SLE and the mechanisms responsible for their altered functionality during disease. One of the key enzymes that control monocyte and DC function is haem oxygenase-1 (HO-1), which catalyses the degradation of the haem group into biliverdin, carbon monoxide and free iron. These products possess immunosuppressive and anti-inflammatory capacities. The main goal of this work was to determine HO-1 expression in monocytes and DCs from patients with SLE and healthy controls. Hence, peripheral blood mononuclear cells were obtained from 43 patients with SLE and 30 healthy controls. CD14(+) monocytes and CD4(+) T cells were sorted by FACS and HO-1 expression was measured by RT-PCR. In addition, HO-1 protein expression was determined by FACS. HO-1 levels in monocytes were significantly reduced in patients with SLE compared with healthy controls. These results were confirmed by flow cytometry. No differences were observed in other cell types, such as DCs or CD4(+) T cells, although decreased MHC-II levels were observed in DCs from patients with SLE. In conclusion, we found a significant decrease in HO-1 expression, specifically in monocytes from patients with SLE, suggesting that an imbalance of monocyte function could be partly the result of a decrease in HO-1 expression.
Authors:
Andrés A Herrada; Carolina Llanos; Juan P Mackern-Oberti; Leandro J Carreño; Carla Henriquez; Roberto S Gómez; Miguel A Gutierrez; Ignacio Anegon; Sergio H Jacobelli; Alexis M Kalergis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  136     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-03     Completed Date:  2012-09-11     Revised Date:  2013-08-14    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  414-24     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Immunology © 2012 Blackwell Publishing Ltd.
Affiliation:
Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Antigens, CD14 / biosynthesis
Arthritis, Rheumatoid / immunology,  pathology
CD4-Positive T-Lymphocytes / enzymology,  immunology
Dendritic Cells / enzymology*,  immunology
Female
Heme Oxygenase-1 / metabolism*
Humans
Lupus Erythematosus, Systemic / enzymology*,  immunology*
Lymphocyte Activation
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Middle Aged
Monocytes / enzymology*,  immunology
RNA, Messenger / genetics,  metabolism
Receptors, IgG / genetics,  immunology
Young Adult
Chemical
Reg. No./Substance:
0/Antigens, CD14; 0/Fcgr2b protein, mouse; 0/RNA, Messenger; 0/Receptors, IgG; EC 1.14.99.3/Heme Oxygenase-1
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