| Heme oxygenase expression in selected regions of term human placenta. | |
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MedLine Citation:
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PMID: 12709587 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Carbon monoxide (CO), formed during heme oxygenase (HO)-catalyzed oxidation of heme, has been proposed to play a complementary role with nitric oxide in the regulation of placental hemodynamics. The objective of this study was to elucidate HO enzymatic activity and HO-1 (inducible) and HO-2 (constitutive) protein content in the microsomal subcellular fraction of homogenate of selected regions of placenta from normotensive and mild pre-eclamptic pregnancies. HO enzymatic activity was measured under optimized conditions by gas chromatography using CO formation as an index of activity, and HO-1 and HO-2 protein content were determined by Western immunoblot analysis. Microsomal HO activity in each of the four placental regions was not different between normotensive and mild pre-eclamptic pregnancies. Microsomal HO-2 protein content was not different between normotensive and mild pre-eclamptic pregnancies, whereas there was increased expression of microsomal HO-1 protein in chorionic villi and fetal membranes from pre-eclamptic pregnancy compared with normotensive pregnancy. Microsomal HO enzymatic activity correlated with HO-2, but not HO-1, protein content. |
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Authors:
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Brian E McLaughlin; Gendie E Lash; Graeme N Smith; Gerald S Marks; Kanji Nakatsu; Charles H Graham; James F Brien |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental biology and medicine (Maywood, N.J.) Volume: 228 ISSN: 1535-3702 ISO Abbreviation: Exp. Biol. Med. (Maywood) Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-04-23 Completed Date: 2003-06-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100973463 Medline TA: Exp Biol Med (Maywood) Country: United States |
Other Details:
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Languages: eng Pagination: 564-7 Citation Subset: IM |
Affiliation:
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Departments of Pharmacology, Faculty of Health Sciences, Queen's University, Kingston, Ontario, Canada K7L 3N6. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Fractionation Female Heme Oxygenase (Decyclizing) / metabolism* Heme Oxygenase-1 Humans Membrane Proteins Microsomes / enzymology Placenta / enzymology* Pre-Eclampsia / metabolism* Pregnancy |
| Chemical | |
Reg. No./Substance:
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0/Membrane Proteins; EC 1.14.99.3/HMOX1 protein, human; EC 1.14.99.3/Heme Oxygenase (Decyclizing); EC 1.14.99.3/Heme Oxygenase-1; EC 1.14.99.3/heme oxygenase-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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