Document Detail

Heme oxygenase expression in selected regions of term human placenta.
MedLine Citation:
PMID:  12709587     Owner:  NLM     Status:  MEDLINE    
Carbon monoxide (CO), formed during heme oxygenase (HO)-catalyzed oxidation of heme, has been proposed to play a complementary role with nitric oxide in the regulation of placental hemodynamics. The objective of this study was to elucidate HO enzymatic activity and HO-1 (inducible) and HO-2 (constitutive) protein content in the microsomal subcellular fraction of homogenate of selected regions of placenta from normotensive and mild pre-eclamptic pregnancies. HO enzymatic activity was measured under optimized conditions by gas chromatography using CO formation as an index of activity, and HO-1 and HO-2 protein content were determined by Western immunoblot analysis. Microsomal HO activity in each of the four placental regions was not different between normotensive and mild pre-eclamptic pregnancies. Microsomal HO-2 protein content was not different between normotensive and mild pre-eclamptic pregnancies, whereas there was increased expression of microsomal HO-1 protein in chorionic villi and fetal membranes from pre-eclamptic pregnancy compared with normotensive pregnancy. Microsomal HO enzymatic activity correlated with HO-2, but not HO-1, protein content.
Brian E McLaughlin; Gendie E Lash; Graeme N Smith; Gerald S Marks; Kanji Nakatsu; Charles H Graham; James F Brien
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  228     ISSN:  1535-3702     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-23     Completed Date:  2003-06-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  564-7     Citation Subset:  IM    
Departments of Pharmacology, Faculty of Health Sciences, Queen's University, Kingston, Ontario, Canada K7L 3N6.
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MeSH Terms
Cell Fractionation
Heme Oxygenase (Decyclizing) / metabolism*
Heme Oxygenase-1
Membrane Proteins
Microsomes / enzymology
Placenta / enzymology*
Pre-Eclampsia / metabolism*
Reg. No./Substance:
0/Membrane Proteins; EC protein, human; EC Oxygenase (Decyclizing); EC Oxygenase-1; EC oxygenase-2

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