Document Detail


Heme oxygenase expression as a biomarker of exposure to amphiphilic polymer-coated CdSe/ZnS quantum dots.
MedLine Citation:
PMID:  22264017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Because of their unique optical properties, quantum dots (QDs) have become a preferred system for ultrasensitive detection and imaging. However, since QDs commonly contain Cd and other heavy metals, concerns have been raised regarding their toxicity. QDs are thus commonly synthesised with a ZnS cap structure and/or coated with polymeric stabilisers. We recently synthesised amphiphilic polymer-coated tri-n-octylphosphine oxide - poly(maleic anhydride-alt-1-tetradecene (TOPO-PMAT) QDs, which are highly stable in aqueous environments. The effects of these QDs on viability and stress response in five cell lines of mouse and human origins are reported here. Human and mouse macrophages and human kidney cells readily internalised these QDs, resulting in modest toxicity. TOPO-PMAT QD exposure was highly correlated with the induction of the stress response protein heme oxygenase-1 (HMOX1). Other stress biomarkers (glutamate cysteine ligase modifier subunit, NAD(P)H, necrosis) were only moderately affected. HMOX1 may thus be a useful biomarker of TOPO-QDOT QD exposure across cell types and species.
Authors:
Lisa A McConnachie; Collin C White; Dianne Botta; Megan E Zadworny; David P Cox; Richard P Beyer; Xiaoge Hu; David L Eaton; Xiaohu Gao; Terrance J Kavanagh
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-01-20
Journal Detail:
Title:  Nanotoxicology     Volume:  7     ISSN:  1743-5404     ISO Abbreviation:  Nanotoxicology     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-05     Completed Date:  2013-07-15     Revised Date:  2014-07-08    
Medline Journal Info:
Nlm Unique ID:  101233132     Medline TA:  Nanotoxicology     Country:  England    
Other Details:
Languages:  eng     Pagination:  181-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Blotting, Western
Cadmium Compounds / metabolism,  toxicity*
Cell Line
Cell Survival / drug effects
Cluster Analysis
Dose-Response Relationship, Drug
Heme Oxygenase-1 / metabolism*
Humans
Lipid Peroxidation / drug effects
Membrane Proteins / metabolism*
Mice
Microscopy, Confocal
Necrosis
Organophosphorus Compounds / toxicity
Oxidative Stress / drug effects
Polymers / metabolism,  toxicity*
Quantum Dots*
Selenium Compounds / metabolism,  toxicity*
Sulfhydryl Compounds / metabolism
Sulfides / metabolism,  toxicity*
Surface-Active Agents / metabolism,  toxicity*
Zinc Compounds / metabolism,  toxicity*
Grant Support
ID/Acronym/Agency:
P30 ES007033/ES/NIEHS NIH HHS; P30ES07033/ES/NIEHS NIH HHS; R01 ES016189/ES/NIEHS NIH HHS; R01ES016189/ES/NIEHS NIH HHS; T32 ES007032/ES/NIEHS NIH HHS; T32ES007032/ES/NIEHS NIH HHS; U19 ES019545/ES/NIEHS NIH HHS; U19ES019545/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Cadmium Compounds; 0/Membrane Proteins; 0/Organophosphorus Compounds; 0/Polymers; 0/Selenium Compounds; 0/Sulfhydryl Compounds; 0/Sulfides; 0/Surface-Active Agents; 0/Zinc Compounds; 0/poly(1-tetradecene-alt-maleic acid); 78-50-2/trioctyl phosphine oxide; A7F646JC5C/cadmium selenide; EC 1.14.99.3/HMOX1 protein, human; EC 1.14.99.3/Heme Oxygenase-1; EC 1.14.99.3/Hmox1 protein, mouse; KPS085631O/zinc sulfide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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