| Heme oxygenase-1 mediates protective effects on inflammatory, catabolic and senescence responses induced by interleukin-1β in osteoarthritic osteoblasts. | |
| | |
MedLine Citation:
|
PMID: 22155307 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Osteoarthritis (OA) is a chronic degenerative joint disease showing altered bone metabolism. Osteoblasts contribute to the regulation of cartilage metabolism and bone remodeling. We have shown previously that induction of heme oxygenase-1 (HO-1) protects OA cartilage against inflammatory and degradative responses. In this study, we investigated the effects of HO-1 induction on OA osteoblast metabolism. HO-1 was induced with cobalt protoporphyrin IX (CoPP) and by transduction with LV-HO-1. In osteoblasts stimulated with interleukin (IL)-1β, CoPP enhanced mineralization, the expression of a number of markers of osteoblast differentiation such as Runx2, bone morphogenetic protein-2, osteocalcin, and collagen 1A1 and 1A2, as well as the ratio osteoprotegerin/receptor activator of nuclear factor-κB ligand. HO-1 induction significantly reduced the expression of matrix metalloproteinase (MMP)-1, MMP-2 and MMP-3, and the production of pro-inflammatory cytokines such as tumor necrosis factor-α and IL-6 whereas IL-10 levels increased. HO-1 also exerted inhibitory effects on prostaglandin (PG)E(2) production which could be dependent on cyclooxygenase-2 and microsomal PGE synthase-1 down-regulation. The activity of senescence-associated β-galactosidase and the expression of the senescence marker caveolin-1 were significantly decreased after HO-1 induction. The inhibition of nuclear factor-κB activation induced by IL-1β in OA osteoblasts may contribute to some HO-1 effects. Our results have shown that HO-1 decreases the production of relevant inflammatory and catabolic mediators that participate in OA pathophysiology thus eliciting protective effects in OA osteoblasts. |
| | |
Authors:
|
Victoria Clérigues; Maria Isabel Guillén; Miguel Angel Castejón; Francisco Gomar; Vicente Mirabet; Maria José Alcaraz |
Related Documents
:
|
15046197 - Molecular actions of selenium in the brain: neuroprotective mechanisms of an essential ... 20967887 - Wnt signaling in activated microglia is proinflammatory. 20486807 - Minozac treatment prevents increased seizure susceptibility in a mouse "two-hit" model ... 10619437 - Mechanisms of brain-mediated systemic anti-inflammatory syndrome causing immunodepression. 20060927 - Exacerbation of tobacco smoke mediated apoptosis by resveratrol: an unexpected conseque... 3017577 - Stimulatory effects of purified macrophage colony-stimulating factor on murine resident... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-2 |
Journal Detail:
|
Title: Biochemical pharmacology Volume: - ISSN: 1873-2968 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0101032 Medline TA: Biochem Pharmacol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
|
Department of Pharmacology, University of Valencia, Burjasot, 46100 Valencia, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: WITHDRAWN: Adenoviral gene delivery can convert adult human fibroblasts to neurons.
Next Document: Inactivation of a class A and a class C ?-lactamase by 6?-(hydroxymethyl)penicillanic acid sulfone.