Document Detail


Hematological and hemostaseological alterations after warm and cold limb ischemia-reperfusion in a canine model.
MedLine Citation:
PMID:  19851685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Acute ischemia-reperfusion (I/R) of extremities means serious challenge in the clinical practice. Furthermore, the issue of preventive cooling is still controversial. In this canine model we investigated whether limb I/R -with or without cooling- has an influence on hematological and hemostaseological factors.
METHODS: Femoral vessels were exposed and clamped for 3 hours. After release the clamps, 4-hour reperfusion was secured. The same procedures with cooling using ice bags, as well as warm and cold sham-operations were performed. Before operations, from the excluded limb by the end of ischemia, during the reperfusion, and for 5 postoperative days afterwards blood samples were collected for testing hematological and blood coagulation parameters.
RESULTS: After I/R activated partial thromboplastin time was elongated on 2nd-4th postoperative days. The highest values were on the 2nd day in cold I/R group, accompanied by increased prothrombin time values. The hematological parameters and fibrinogen level showed non-specific changes. In excluded ischemic limb the blood composition showed controversial data. Cold ischemia induced larger alterations, however platelet count, hematocrit changed more expressly in warm ischemia.
CONCLUSION: These results indicate the risk of coagulopathy following limb I/R on early post-eventually days, which risk is higher in the case of cold I/R.
Authors:
Miklos Szokoly; Norbert Nemeth; Istvan Furka; Iren Miko
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta cirúrgica brasileira / Sociedade Brasileira para Desenvolvimento Pesquisa em Cirurgia     Volume:  24     ISSN:  1678-2674     ISO Abbreviation:  Acta Cir Bras     Publication Date:    2009 Sep-Oct
Date Detail:
Created Date:  2009-10-23     Completed Date:  2010-11-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9103983     Medline TA:  Acta Cir Bras     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  338-46     Citation Subset:  IM    
Affiliation:
National Institute of Traumatology, Budapest.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Coagulation Disorders / blood*,  etiology
Body Temperature
Cold Temperature / adverse effects*
Disease Models, Animal
Dogs
Fibrinogen / analysis
Hemorheology
Hemostasis
Hindlimb / blood supply*
Ischemia / blood*
Lower Extremity / blood supply*
Random Allocation
Reperfusion Injury / blood*,  complications
Chemical
Reg. No./Substance:
9001-32-5/Fibrinogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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