Document Detail

Hematologic and biochemical effects of the Gradiflow in an ex vivo ovine model.
MedLine Citation:
PMID:  16156300     Owner:  NLM     Status:  MEDLINE    
The Gradiflow (Life Therapeutics, Frenchs Forest, Australia) system is a novel electrophoretic technique that uses the dual characteristics of size and charge to separate target macromolecules from complex biological solutions. The system has the potential to selectively remove a range of moieties from blood and plasma in an in vivo system such as hemodialysis or, alternatively, in an in vitro setting such as a blood bank. In this study, the safety of a scaled down Gradiflow prototype device with a membrane surface area of 16 cm2 was investigated using an ex vivo ovine model. Physiologic, hematologic, and biochemical parameters were assessed in 12 sheep: 6 animals treated using the Gradiflow and 6 controls. The effects of the Gradiflow procedure on both whole blood and plasma were analyzed. The Gradiflow procedure was well tolerated, and the application of an electrical potential or exposure to the Gradiflow membrane did not cause any significant changes in the parameters measured. Hemoglobin levels remained stable in all groups during the 4-hour experiment. An early neutropenia was observed in all groups, although this appeared to be more pronounced with exposure to a plasma filter; the presence of the Gradiflow component had no separate influence. One sheep in the plasma group experienced septic shock, caused by Staphylococcus contamination of the separation membrane. Overall, the results indicate that there were no gross physiologic, hematologic, or biochemical adverse reactions to the ex vivo Gradiflow procedure.
Jason I Lee; Anne Simmons; Ross O Odell; Steven A Botto; Ellie L Faramus; Klaus Schindhelm; Philip J Roeth; Hari C Nair; David C H Harris
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  ASAIO journal (American Society for Artificial Internal Organs : 1992)     Volume:  51     ISSN:  1058-2916     ISO Abbreviation:  ASAIO J.     Publication Date:    2005 Jul-Aug
Date Detail:
Created Date:  2005-09-13     Completed Date:  2005-10-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9204109     Medline TA:  ASAIO J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  366-71     Citation Subset:  IM    
Department of Renal Medicine, The University of Sydney at Westmead Millennium Institute, Westmead, Australia.
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MeSH Terms
Aspartate Aminotransferases / blood
Bilirubin / blood
Blood Chemical Analysis
Blood Pressure
Body Temperature
Creatine Kinase / blood
Electrophoresis, Polyacrylamide Gel / instrumentation*,  veterinary*
Extracorporeal Circulation
Heart Rate
Hemoglobins / analysis
Leukocyte Count
Plasma / chemistry
Renal Dialysis / instrumentation*,  veterinary*
Time Factors
Reg. No./Substance:
0/Hemoglobins; 635-65-4/Bilirubin; EC Aminotransferases; EC Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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