Document Detail


Hemangiogenesis and lymphangiogenesis in corneal pathology.
MedLine Citation:
PMID:  22030600     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: We characterized the presence of hemangiogenesis (HA) and lymphangiogenesis (LA) in human corneal specimens exhibiting 13 underlying pathologies.
METHODS: Human corneal specimens were obtained from consenting subjects (n = 2 or n = 3 for each pathology; total sample size, n = 35). The pathological specimens were stained with hematoxylin and eosin (H&E) to determine the presence or absence of corneal neovascularization (NV) and superficial or deep stromal distribution of NV. Immunohistochemical staining was then performed to differentiate HA (positive for CD31) from LA (positive for lymphatic vessel endothelial hyaluronan receptor-1 [LYVE-1]).
RESULTS: The double-negative (CD31(-)/LYVE-1(-)) immunostaining, indicating the absence of NV, was exhibited by 21 specimens (60%). CD31(-)/LYVE-1(-), indicating the presence of HA and absence of LA, was exhibited by 12 specimens (34%). The double-positive (CD31(+)/LYVE-1(+)) phenotype, indicating both HA and LA, was exhibited by 2 specimens (6%). Notably, the CD31(-)/LYVE-1(-) phenotype, indicating the presence of LA and absence of HA, was not detected among the specimens. Deep stromal NV was exhibited in a 4:3 ratio to superficial stromal NV. The double-negative immunostaining was more prevalent in noninflammatory pathologies, particularly in comparison with combined neovascular phenotypes (ie, CD31(+) or LYVE-1(+)). Among the neovascular phenotypes, HA was 7 times more common than LA. Specimens exhibiting LA presented only with the double-positive phenotype.
CONCLUSIONS: HA is the predominant component of NV in corneal pathologies. LA accompanies HA; however, isolated LA (from lymphatics in the conjunctiva) does not occur in these corneal pathologies. Our results suggest the potential therapeutic utility of targeting antineovascular therapies specifically for corneal HA and/or LA pathology.
Authors:
Makambo Tshionyi; Elizabeth Shay; Elisa Lunde; Amy Lin; Kyu-Yeon Han; Sandeep Jain; Jin-Hong Chang; Dimitri T Azar
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cornea     Volume:  31     ISSN:  1536-4798     ISO Abbreviation:  Cornea     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-13     Completed Date:  2012-02-02     Revised Date:  2013-07-17    
Medline Journal Info:
Nlm Unique ID:  8216186     Medline TA:  Cornea     Country:  United States    
Other Details:
Languages:  eng     Pagination:  74-80     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, Chicago, IL, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD31 / metabolism
Cornea / blood supply*,  metabolism
Corneal Neovascularization / immunology,  metabolism,  pathology*
Corneal Stroma / metabolism
Female
Humans
Immunohistochemistry
Lymphangiogenesis*
Lymphatic Vessels / metabolism
Male
Vesicular Transport Proteins / metabolism
Grant Support
ID/Acronym/Agency:
EY01792/EY/NEI NIH HHS; EY10101/EY/NEI NIH HHS; P30 EY001792/EY/NEI NIH HHS; P30 EY001792-34/EY/NEI NIH HHS; R01 EY010101/EY/NEI NIH HHS; R21 EY021886/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD31; 0/LYVE1 protein, human; 0/Vesicular Transport Proteins
Comments/Corrections

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