Document Detail


Helix-coil kinetics of individual polyadenylic acid molecules in a protein channel.
MedLine Citation:
PMID:  20482020     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Helix-coil transition kinetics of polyadenylic acid [poly(A)] inside a small protein channel is investigated for the first time, at the single molecule level. The confinement of a RNA molecule inside the channel slows its kinetics by nearly 3 orders of magnitude as compared to bulk measurements of free poly(A). These findings are related to the interaction energy of the RNA structure with the interior of the pore, explained by a simple two-state model. These results shed light on the way intermolecular interactions alter nucleic acid kinetics.
Authors:
Jianxun Lin; Anatoly Kolomeisky; Amit Meller
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-04-15
Journal Detail:
Title:  Physical review letters     Volume:  104     ISSN:  1079-7114     ISO Abbreviation:  Phys. Rev. Lett.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-05-20     Completed Date:  2010-08-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401141     Medline TA:  Phys Rev Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  158101     Citation Subset:  IM    
Affiliation:
Department of Physics, Boston University, Boston, Massachusetts 02215, USA.
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MeSH Terms
Descriptor/Qualifier:
Electricity
Hemolysin Proteins / chemistry,  metabolism
Kinetics
Models, Molecular
Molecular Conformation*
Nanostructures / chemistry
Poly A / chemistry*,  metabolism
Porosity
Proteins / chemistry*,  metabolism
RNA / chemistry,  metabolism
Temperature
Thermodynamics
Time Factors
Chemical
Reg. No./Substance:
0/Hemolysin Proteins; 0/Proteins; 24937-83-5/Poly A; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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