Document Detail

Heliocoverpa armigera single nucleocapsid nucleopolyhedrovirus induces Hz-AM1 cell cycle arrest at the G2 phase with accumulation of cyclin B1.
MedLine Citation:
PMID:  15351484     Owner:  NLM     Status:  MEDLINE    
The cell cycle phase distributions of Hz-AM1 cells grown in monolayer culture were G1 = 49.7 +/- 3.3%, S = 22.7 +/- 3.8% and G2/M = 27.8 +/- 4.2% without >4N DNA content. The culture doubling time was about 40 h and the duration of the G1, S and G2/M phases was estimated to be 10, 14 and 16 h, respectively. HaSNPV infection of Hz-AM1 cells resulted in both unsynchronized and synchronized G1 phase arrest at G2/M phase. HaSNPV infection also resulted in the appearance of more than 4N DNA content, which accumulated to the highest levels 72 h post-infection. We also found that the expression level of cyclin B1 increased significantly after 16 h post-infection, while cyclin A did not show any change. This observation supports the Hz-AM1-infected arrest at the G2/M phage. Cytoplasm location of cyclin B1 indicated that the Hz-AM1 cell cycle arrest was at the G2 phase.
Rui Zhou; Ze-Hua Yu; Xiao-qing Li; Fang Jia; Jian-hong Wu; Xinwen Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virus research     Volume:  105     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-09-07     Completed Date:  2005-03-11     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  113-20     Citation Subset:  IM    
Institution of Entomology, Central China Normal University, Wuhan 430070, PR China.
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MeSH Terms
Cell Cycle*
Cell Division
Cell Line
Cyclin A / metabolism
Cyclin B / metabolism*
Cyclin B1
Cytoplasm / metabolism
DNA, Viral / analysis
G2 Phase*
Moths / cytology,  metabolism,  virology*
Nucleopolyhedrovirus / physiology*
Time Factors
Reg. No./Substance:
0/Cyclin A; 0/Cyclin B; 0/Cyclin B1; 0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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