| Helicobacter pylori lipopolysaccharide activity in human peripheral blood mononuclear leukocyte cultures. | |
| | |
MedLine Citation:
|
PMID: 20814071 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Helicobacter pylori (H. pylori) have been recognized as a major cause of chronic gastritis, gastric and duodenal ulcers and gastric cancer. Macrophages are the targets of lipopolysaccharide (LPS), which is a constituent of the outer membrane of Gram-negative rods. In this study we focused on a potential role of macrophages in the proliferation of human peripheral blood mononuclear leukocytes (PBML) in the milieu of H. pylori LPS and standard E. coli LPS. First, we found that H. pylori and E. coli LPS induced proliferation of total PBML (tPBML) from 5 out 21 healthy blood donors (LPS responders). In the LPS milieu, tPBML from the majority of volunteers (LPS non-responders) showed a significant decrease in the [(3)H]-thymidine incorporation as compared to tPBML in medium alone. The decreased cell proliferation was associated with a diminished metabolic activity of non-adherent lymphocytes. Then, non-adherent lymphocytes were stimulated with autologous macrophages pulsed with bacterial LPS. Still, the lymphocytes from the non-responders did not proliferate in the cultures with LPS exposed macrophages. In the group of LPS responders, the macrophages pulsed with H. pylori LPS significantly reduced the proliferation of non-adherent lymphocytes. The possible mechanism regulating the responses of PBML to bacterial LPS with an implication for the outcome of H. pylori infections is discussed. |
| | |
Authors:
|
A Grebowska; A P Moran; W Bielanski; A Matusiak; T Rechcinski; K Rudnicka; A Baranowska; W Rudnicka; M Chmiela |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society Volume: 61 ISSN: 1899-1505 ISO Abbreviation: J. Physiol. Pharmacol. Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-09-03 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9114501 Medline TA: J Physiol Pharmacol Country: Poland |
Other Details:
|
Languages: eng Pagination: 437-42 Citation Subset: IM |
Affiliation:
|
Department of Immunology and Infectious Biology, University of Lodz, Lodz, Poland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Gastric ulcer healing and stress-lesion preventive properties of pioglitazone are attenuated in diab...
Next Document: Importance of ghrelin in hypothalamus - pituitary axis on growth hormone release during normal pregn...