Document Detail


Helicobacter pylori increases the epithelial permeability to a food antigen in human gastric biopsies.
MedLine Citation:
PMID:  15046209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: We have previously shown that H. pylori increases the passage of intact protein across the digestive epithelium, in vitro and in vivo in mice. The aim of this study was to test whether H. pylori can alter gastric permeability to a food-type antigen in humans. METHODS: Antral and corpus gastric biopsies obtained from H. pylori-positive and H. pylori-negative patients suffering from nonulcer dyspepsia with normal esogastroduodenal endoscopy were mounted in adapted Ussing chambers to measure gastric permeability. Electrical resistance (R, an index of epithelial integrity) and fluxes of 14C-mannitol (JMan, a marker of paracellular permeability) and 3H-horseradish peroxidase (HRP, a test protein of antigen transport and processing) under intact (JHRPi) and degraded (JD) form, across the biopsies, were measured. RESULTS: At the corpus level, biopsies obtained from H. pylori-positive patients, as compared to H. pylori-negative patients, presented significantly higher intact--but not degraded--HRP fluxes (JHRPi = 446 +/- 297 and 219 +/- 265 ng/h cm2, p < 0.05; JD = 4,247 +/- 3,884 and 3,575 +/- 2,594 ng/h x cm2, respectively), and an increase in paracellular permeability (Jman = 0.35 +/- 0.1 vs 0.24 +/- 0.08 micromol/h x cm2, p < 0.02, respectively). At the antrum level, a tendency to increased intact HRP fluxes in H. pylori-positive patients, as compared to H. pylori-negative patients, was observed (JHRPi = 284 +/- 250 vs 207 +/- 203 ng/h x cm2, p < 0.09), without modifications of paracellular permeability. CONCLUSIONS: H. pylori infection increases absorption of an intact food antigen across the corpus gastric mucosa. This phenomenon may contribute to the maintenance of gastric inflammation and could play a role in the development of allergic sensitization to dietary antigens in susceptible individuals.
Authors:
Tamara Matysiak-Budnik; Benoît Coffin; Anne Lavergne-Slove; Jean-Marc Sabate; Francis Mégraud; Martine Heyman
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The American journal of gastroenterology     Volume:  99     ISSN:  0002-9270     ISO Abbreviation:  Am. J. Gastroenterol.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-03-29     Completed Date:  2004-05-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0421030     Medline TA:  Am J Gastroenterol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  225-32     Citation Subset:  IM    
Affiliation:
INSERM EMI-0212, Faculté de Médecine, Necker-Enfants Malades, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Antigens / immunology*
Biopsy
Cell Membrane Permeability / immunology*
Dyspepsia / immunology,  microbiology,  pathology
Female
Food
Gastric Mucosa / immunology*,  microbiology,  pathology
Helicobacter Infections / immunology*
Helicobacter pylori / immunology*
Horseradish Peroxidase / pharmacology
Humans
Male
Mannitol / pharmacology
Middle Aged
Chemical
Reg. No./Substance:
0/Antigens; 69-65-8/Mannitol; EC 1.11.1.-/Horseradish Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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