| Helicobacter pylori gamma-glutamyl transpeptidase is a pathogenic factor in the development of peptic ulcer disease. | |
| | |
MedLine Citation:
|
PMID: 20347814 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND & AIMS: gamma-Glutamyl transpeptidase (GGT) has been reported to be a virulence factor of Helicobacter pylori associated with bacterial colonization and cell apoptosis. But its mechanism of pathogenesis is not firmly established. This study aims to examine its role in H pylori-mediated infection. METHODS: Various H pylori isogenic mutants were constructed by a polymerase chain reaction (PCR) approach. H pylori native GGT protein (HP-nGGT) was purified with ion-exchange and gel-filtration chromatography. Generation of H2O2 was measured with fluorimetric analysis, whereas nuclear factor-kappaB (NF-kappaB) activation was determined by luciferase assay and Western blot. Cytokine production was examined by enzyme-linked immunoabsorbent assay and real-time PCR. DNA damage was assessed with comet assay and flow cytometry. The GGT activity of 98 H pylori isolates was analyzed by an enzymatic assay. RESULTS: Purified HP-nGGT generated H2O2 in primary gastric epithelial cells and AGS gastric cancer cells, resulting in the activation of NF-kappaB and up-regulation of interleukin-8 (IL-8) production. In addition, HP-nGGT caused an increase in the level of 8-OH-dG, indicative of oxidative DNA damage. In contrast, Deltaggt showed significantly reduced levels of H2O2 generation, IL-8 production, and DNA damage in cells compared with the wild type (P<.05). The clinical importance of GGT was indicated by significantly higher (P<.001) activity in H pylori isolates obtained from patients with peptic ulcer disease (n=54) than isolates from patients with nonulcer dyspepsia (n=44). CONCLUSION: Our findings provide evidence that GGT is a pathogenic factor associated with H pylori-induced peptic ulcer disease. |
| | |
Authors:
|
Min Gong; Samantha Shi Min Ling; Sook Yin Lui; Khay Guan Yeoh; Bow Ho |
Related Documents
:
|
12485124 - Significance of the caspase family in helicobacter pylori induced gastric epithelial ap... 1358524 - Effect of galleria mellonella larvae preparation and honeybee products on cell cultures. 11570524 - T-cell-mediated mucosal immunity in the absence of antibody: lessons from helicobacter ... 1912434 - The sydney system: auto-immune gastritis. 21878544 - Macrophage phagocytosis: effects of environmental pollutants, alcohol, cigarette smoke,... 15623664 - Mechanism of action of mitoxantrone. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-27 |
Journal Detail:
|
Title: Gastroenterology Volume: 139 ISSN: 1528-0012 ISO Abbreviation: Gastroenterology Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-07-27 Completed Date: 2010-08-12 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: United States |
Other Details:
|
Languages: eng Pagination: 564-73 Citation Subset: AIM; IM |
Copyright Information:
|
Copyright (c) 2010 AGA Institute. Published by Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis Bacterial Proteins / genetics, metabolism* Biopsy Blotting, Western Cells, Cultured Chromatography, Gel Chromatography, Ion Exchange Comet Assay DNA Damage Enzyme-Linked Immunosorbent Assay Flow Cytometry Fluorometry Gastric Mucosa / immunology, microbiology*, pathology Helicobacter Infections / complications, microbiology*, pathology Helicobacter pylori / enzymology, genetics, pathogenicity* Humans Hydrogen Peroxide / metabolism Interleukin-8 / genetics, metabolism Mutation NF-kappa B / metabolism Oxidative Stress Reverse Transcriptase Polymerase Chain Reaction Stomach Ulcer / immunology, microbiology*, pathology Time Factors Virulence Factors / genetics, metabolism* gamma-Glutamyltransferase / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/IL8 protein, human; 0/Interleukin-8; 0/NF-kappa B; 0/Virulence Factors; 7722-84-1/Hydrogen Peroxide; EC 2.3.2.2/gamma-Glutamyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Esophageal squamous cell dysplasia and delayed differentiation with deletion of krüppel-like factor...
Next Document: Inhibitor of kappaB kinase beta regulates gastric carcinogenesis via interleukin-1alpha expression.