Document Detail


Helicobacter pylori-derived neutrophil-activating protein increases the lifespan of monocytes and neutrophils.
MedLine Citation:
PMID:  20070310     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An invariable feature of Helicobacter pylori-infected gastric mucosa is the persistent infiltration of inflammatory cells. The neutrophil-activating protein (HP-NAP) has a pivotal role in triggering and orchestrating the phlogistic process associated with H. pylori infection. Aim of this study was to address whether HP-NAP might further contribute to the inflammation by increasing the lifespan of inflammatory cells. We report that HP-NAP is able to prolong the lifespan of monocytes, in parallel with the induction of the anti-apoptotic proteins A1, Mcl-1, Bcl-2 and Bcl-X(L). This effect does not result from a direct action on the apoptotic machinery, but rather it requires the release of endogenous pro-survival factors, such as interleukin-1beta, which probably acts in synergy with other unidentified mediators. We also report that HP-NAP promotes the survival of Ficoll-purified neutrophils in a monocyte-dependent fashion: indeed, mononuclear cell depletion of Ficoll-purified neutrophils completely abolished the pro-survival effect by HP-NAP. In conclusion, our data reinforce the notion that HP-NAP has a pivotal role in sustaining a prolonged activation of myeloid cells.
Authors:
Andrea Cappon; Chiara Babolin; Daniela Segat; Laila Cancian; Amedeo Amedei; Federica Calzetti; Marco A Cassatella; Mario M D'Elios; Marina de Bernard
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-11
Journal Detail:
Title:  Cellular microbiology     Volume:  12     ISSN:  1462-5822     ISO Abbreviation:  Cell. Microbiol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-18     Completed Date:  2010-09-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  754-64     Citation Subset:  IM    
Affiliation:
Venetian Institute of Molecular Medicine, Padua, Italy.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / immunology*
Cell Survival
Cells, Cultured
Helicobacter pylori / immunology*
Humans
Monocytes / immunology*,  microbiology*
Neutrophils / immunology*,  microbiology*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/neutrophil-activating protein A, Helicobacter pylori

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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