|Helicobacter pylori cagA 12-bp insertion can be a marker for duodenal ulcer in Okinawa, Japan.|
|PMID: 23190390 Owner: NLM Status: MEDLINE|
|BACKGROUND AND AIM: Helicobacter pylori cagA can be classified into mainly two types (East-Asian-type and Western-type cagA) according to the repeat regions located in the 3' region. Recent studies showed that the Western-type cagA in strains from Okinawa, Japan formed a different cluster (J-Western-type cagA subtype). It has also been reported that J-Western-type cagA possesses a 12-bp insertion located in the 5' region of cagA sequence.
METHODS: The prevalence of 12-bp insertion in cagA in Okinawa and the United States (U.S.) was examined by DNA sequencing. The primer pair that can detect the 12-bp insertion only by polymerase chain reaction was then designed. The prevalence of strains with 12-bp insertion was examined in 336 strains isolated from Okinawa by polymerase chain reaction.
RESULTS: In case of Western-type cagA/vacA s1m2 strains, the prevalence of 12-bp insertion was significantly higher in strains isolated from Okinawa than that from the U.S. (P = 0.002). Phylogenetic tree showed that strains with 12-bp insertion formed two individual clusters within J-Western-type cagA subtype; one is from Okinawa and another is from the U.S. The designed primer set showed high sensitivity (100%) and specificity (90.8%) in Okinawa. The 12-bp insertion was found in 23.7%, 14.3%, 4.2%, and 4.0% of strains with duodenal ulcer (DU), gastritis, gastric cancer, and gastric ulcer (GU), respectively (P < 0.001 for DU vs GU) in Okinawa.
CONCLUSIONS: Although the mechanisms are unknown, the presence of 12-bp insertion was associated with the presence of DU and might have a suppressive action on GU and gastric cancer.
|Yuichi Matsuo; Seiji Shiota; Osamu Matsunari; Rumiko Suzuki; Masahide Watada; Tran Thanh Binh; Nagisa Kinjo; Fukunori Kinjo; Yoshio Yamaoka|
|Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't|
|Title: Journal of gastroenterology and hepatology Volume: 28 ISSN: 1440-1746 ISO Abbreviation: J. Gastroenterol. Hepatol. Publication Date: 2013 Feb|
|Created Date: 2013-01-23 Completed Date: 2013-07-26 Revised Date: 2014-02-04|
Medline Journal Info:
|Nlm Unique ID: 8607909 Medline TA: J Gastroenterol Hepatol Country: Australia|
|Languages: eng Pagination: 291-6 Citation Subset: IM|
|© 2012 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd.|
|APA/MLA Format Download EndNote Download BibTex|
Bacterial Proteins / genetics*
DNA Mutational Analysis
DNA, Bacterial / analysis*
Duodenal Ulcer / diagnosis*, epidemiology, microbiology
Gastritis / diagnosis, microbiology
Helicobacter Infections / diagnosis*, epidemiology, microbiology
Helicobacter pylori / classification, genetics*
Japan / epidemiology
Polymerase Chain Reaction
Predictive Value of Tests
Stomach Neoplasms / diagnosis, microbiology
Stomach Ulcer / diagnosis, microbiology
United States / epidemiology
|DK62813/DK/NIDDK NIH HHS; R01 DK062813/DK/NIDDK NIH HHS|
|0/Antigens, Bacterial; 0/Bacterial Proteins; 0/DNA Primers; 0/DNA, Bacterial; 0/Genetic Markers; 0/cagA protein, Helicobacter pylori|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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