Document Detail

Helicobacter pylori cagA 12-bp insertion can be a marker for duodenal ulcer in Okinawa, Japan.
MedLine Citation:
PMID:  23190390     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND AIM: Helicobacter pylori cagA can be classified into mainly two types (East-Asian-type and Western-type cagA) according to the repeat regions located in the 3' region. Recent studies showed that the Western-type cagA in strains from Okinawa, Japan formed a different cluster (J-Western-type cagA subtype). It has also been reported that J-Western-type cagA possesses a 12-bp insertion located in the 5' region of cagA sequence.
METHODS: The prevalence of 12-bp insertion in cagA in Okinawa and the United States (U.S.) was examined by DNA sequencing. The primer pair that can detect the 12-bp insertion only by polymerase chain reaction was then designed. The prevalence of strains with 12-bp insertion was examined in 336 strains isolated from Okinawa by polymerase chain reaction.
RESULTS: In case of Western-type cagA/vacA s1m2 strains, the prevalence of 12-bp insertion was significantly higher in strains isolated from Okinawa than that from the U.S. (P = 0.002). Phylogenetic tree showed that strains with 12-bp insertion formed two individual clusters within J-Western-type cagA subtype; one is from Okinawa and another is from the U.S. The designed primer set showed high sensitivity (100%) and specificity (90.8%) in Okinawa. The 12-bp insertion was found in 23.7%, 14.3%, 4.2%, and 4.0% of strains with duodenal ulcer (DU), gastritis, gastric cancer, and gastric ulcer (GU), respectively (P < 0.001 for DU vs GU) in Okinawa.
CONCLUSIONS: Although the mechanisms are unknown, the presence of 12-bp insertion was associated with the presence of DU and might have a suppressive action on GU and gastric cancer.
Yuichi Matsuo; Seiji Shiota; Osamu Matsunari; Rumiko Suzuki; Masahide Watada; Tran Thanh Binh; Nagisa Kinjo; Fukunori Kinjo; Yoshio Yamaoka
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of gastroenterology and hepatology     Volume:  28     ISSN:  1440-1746     ISO Abbreviation:  J. Gastroenterol. Hepatol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-23     Completed Date:  2013-07-26     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  8607909     Medline TA:  J Gastroenterol Hepatol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  291-6     Citation Subset:  IM    
Copyright Information:
© 2012 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd.
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MeSH Terms
Antigens, Bacterial / genetics*
Bacterial Proteins / genetics*
Chi-Square Distribution
DNA Mutational Analysis
DNA Primers
DNA, Bacterial / analysis*
Duodenal Ulcer / diagnosis*,  epidemiology,  microbiology
Gastritis / diagnosis,  microbiology
Genetic Markers
Helicobacter Infections / diagnosis*,  epidemiology,  microbiology
Helicobacter pylori / classification,  genetics*
Japan / epidemiology
Logistic Models
Middle Aged
Multivariate Analysis
Mutagenesis, Insertional*
Odds Ratio
Polymerase Chain Reaction
Predictive Value of Tests
Stomach Neoplasms / diagnosis,  microbiology
Stomach Ulcer / diagnosis,  microbiology
United States / epidemiology
Grant Support
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Proteins; 0/DNA Primers; 0/DNA, Bacterial; 0/Genetic Markers; 0/cagA protein, Helicobacter pylori

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