Document Detail


Helicobacter bilis colonization of the biliary system in patients with pancreaticobiliary maljunction.
MedLine Citation:
PMID:  20155791     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Helicobacter bilis is considered to be a causative factor in the pathogenesis of biliary cancer. This study investigated the prevalence of H. bilis colonization of the biliary system of patients with pancreaticobiliary maljunction (PBM). METHODS: Bile juice and biliary tissue samples were collected from 17 patients with PBM and 27 controls who had benign biliary disease without PBM. DNA extracted from each biliary sample was subjected to polymerase chain reaction (PCR) analysis for H. bilis and Helicobacter pylori. RESULTS: PCR assays revealed that 12 of the 17 patients with PBM were positive for H. bilis DNA, compared with eight of 27 patients without PBM (P = 0.009). Among patients with PBM, H. bilis DNA was identified in six of eight children, including a 2-month-old infant, and in six of nine adults. The high prevalence of H. bilis DNA in the biliary system of patients with PBM was independent of age, sex, common bile duct dilatation, configuration of the pancreatic and bile ducts, and amylase activity in bile. CONCLUSION: H. bilis colonization of the biliary system is extremely common in patients with PBM. This may point to a role in the pathogenesis of biliary cancer.
Authors:
T Kosaka; Y Tajima; T Kuroki; T Mishima; T Adachi; N Tsuneoka; K Fukuda; T Kanematsu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The British journal of surgery     Volume:  97     ISSN:  1365-2168     ISO Abbreviation:  Br J Surg     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-10     Completed Date:  2010-03-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372553     Medline TA:  Br J Surg     Country:  England    
Other Details:
Languages:  eng     Pagination:  544-9     Citation Subset:  AIM; IM    
Copyright Information:
Copyright (c) 2010 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.
Affiliation:
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, Japan. taiichiro1111@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Bile / microbiology
Bile Ducts / abnormalities*
Biliary Tract / microbiology
Biliary Tract Neoplasms / microbiology*
Case-Control Studies
Child
Child, Preschool
Electrophoresis
Female
Helicobacter / genetics,  isolation & purification*
Helicobacter Infections*
Humans
Infant
Male
Middle Aged
Pancreatic Ducts / abnormalities*
Polymerase Chain Reaction
RNA, Bacterial / analysis*
Chemical
Reg. No./Substance:
0/RNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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