| Helicase translocation assay method using avidin and biotinylated nucleotides. | |
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MedLine Citation:
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PMID: 18594773 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Helicase involves many cellular processes that separate double-stranded nucleic acid into single strands. Although it is believed that helicase translocates nucleic acids, it is difficult to show the direct evidence of translocation on nucleic acids. In this study, an avidin-biotinylated nucleotides-based method for helicase translocation assay has been described, and the biochemical assay results have been demonstrated. |
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Authors:
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Jae-Ho Shin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-02 |
Journal Detail:
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Title: Biotechnology letters Volume: 30 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-09-23 Completed Date: 2009-01-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 2007-12 Citation Subset: IM |
Affiliation:
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School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, South Korea. jhshin@knu.ac.kr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Avidin
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chemistry,
metabolism* Biotinylation DNA Helicases / chemistry, metabolism* DNA, Single-Stranded / chemistry, metabolism DNA-Directed DNA Polymerase / chemistry, metabolism Models, Biological Nucleotides / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Single-Stranded; 0/Nucleotides; 1405-69-2/Avidin; EC 2.7.7.7/DNA-Directed DNA Polymerase; EC 3.6.1.-/DNA Helicases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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