| Helicase-like transcription factor confers radiation resistance in cervical cancer through enhancing the DNA damage repair capacity. | |
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MedLine Citation:
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PMID: 20535496 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Helicase-like transcription factor (HLTF) is a member of the SWI/SNF (mating type switching/sucrose non-fermenting) family of ATPases/helicases and also has a RING-finger motif characteristic of ubiquitin ligase proteins. These features have led to suggestions that HLTF functions like yeast Rad5, which promotes replication through DNA lesions via a post-replication repair pathway. However, the function of HLTF in higher eukaryotes is still unknown. Herein, we found the overexpression of HLTF in radiation recurrent human uterine cervical carcinoma tissues when compared to disease free survived patients tissues. In this study, we used RNA interference techniques to investigate the potential function of HLTF in cervical cancer cell line HeLa and found that the cell proliferation was reduced by knockdown (KD) of HLTF. A host-cell reactivation assay showed that the capacity for repair to DNA damage induced by X-ray irradiation was reduced in HLTF KD cells. X-rays also increased apoptosis in HLTF KD cells. These results suggest that HLTF is involved in DNA repair and apoptosis in cancer cells, which might represent a target for gene therapies of human cancer. |
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Authors:
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Sunghwan Cho; Senthilkumar Cinghu; Jae-Ran Yu; Woo-Yoon Park |
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Publication Detail:
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Type: Journal Article Date: 2010-06-10 |
Journal Detail:
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Title: Journal of cancer research and clinical oncology Volume: 137 ISSN: 1432-1335 ISO Abbreviation: J. Cancer Res. Clin. Oncol. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7902060 Medline TA: J Cancer Res Clin Oncol Country: Germany |
Other Details:
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Languages: eng Pagination: 629-37 Citation Subset: IM |
Affiliation:
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Department of Radiation Oncology, Chungbuk National University College of Medicine, Cheongju, 361-763, Republic of Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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