Document Detail


Helianthus tuberosus agglutinin directly induces neutrophil migration, which can be modulated/inhibited by resident mast cells.
MedLine Citation:
PMID:  16234855     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the effect of Helianthus tuberosus agglutinin (HTA) on neutrophil migration in vivo and in vitro. The role of resident cells in this effect was analyzed. Peritonitis was induced by injecting stimuli into rat (150-200 g) peritoneal cavities, and in vitro neutrophil chemotaxis was performed using a Boyden microchamber. HTA (80, 200, or 500 microg/mL per cavity) induced significant in vivo neutrophil migration (p < 0.05); in vitro assays showed that this lectin also induced neutrophil chemotaxis, an effect inhibited by the incubation of lectin associated with alpha-D(+)-mannose, its specific binding sugar. Depletion of the resident-cell population by peritoneal lavage did not alter HTA-induced neutrophil migration (200 microg/mL per cavity). The opposite strategy, increasing peritoneal macrophages by intraperitoneally injecting rats with thioglycollate, did not enhance the neutrophil migration produced by HTA (200 microg/mL per cavity). In addition, injection of supernatant from HTA-stimulated macrophage culture (300 microg/mL) into rat peritoneal cavities did not induce neutrophil migration. However, reduction of the peritoneal mast-cell population potentiated the neutrophil migration (p < 0.05) induced by HTA (200 microg/mL per cavity). Lectin from H. tuberosus has a direct neutrophil chemotatic effect that is modulated by mast cells.
Authors:
Veruska B M Alencar; Gerly A C Brito; Nylane M N Alencar; Ana M S Assreuy; Vicente P T Pinto; Edson H Teixeira; Emmanuel P Souza; Henri Debray; Ronaldo A Ribeiro; Benildo S Cavada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry and cell biology = Biochimie et biologie cellulaire     Volume:  83     ISSN:  0829-8211     ISO Abbreviation:  Biochem. Cell Biol.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-19     Completed Date:  2006-05-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8606068     Medline TA:  Biochem Cell Biol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  659-66     Citation Subset:  IM    
Affiliation:
Dept. de Fisiologia e Farmacologia/Faculdade de Medicina, UniversidadeFederal do Ceará, Rua Coronel Nunes de Melo, 1127 Rodolfo Teófilo 60430-270, Fortaleza, Ceará, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chemotaxis, Leukocyte / drug effects*
Dose-Response Relationship, Drug
Female
Macrophages, Peritoneal / cytology,  drug effects
Male
Mast Cells / cytology*
Neutrophils / cytology,  drug effects*
Plant Lectins / pharmacology*
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Helianthus tuberosus lectin; 0/Plant Lectins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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