Document Detail


Heightened reactive oxygen species generation in the antimicrobial activity of a three component iodinated chitosan-silver nanoparticle composite.
MedLine Citation:
PMID:  20085297     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Herein we report synergy in antimicrobial activity of a chitosan-silver nanoparticle (CS-Ag NP) composite in the presence of molecular iodine. Green fluorescent protein (GFP) expressing recombinant Escherichia coli bacteria have been used to test the efficacy and establish the mechanism of action. Experimental evidence indicate significantly high bactericidal activity of the nanocomposite in the presence of iodine than either due to the composite, chitosan, Ag NP or iodine only. Transmission electron microscopy measurements revealed attachment of bacteria to the composite. In addition, flow cytometry results supported definite occurrence of cell wall damage of the bacteria treated with the composite in the presence of iodine. Further, the nanocomposite and iodine combination was found to exert reactive oxygen species (ROS) generated oxidative stress in the cytoplasm of bacterial cells, leading to cell death. Elucidation of the mechanism of synergy due to three potential antibacterial components suggested that on the surface of Ag NPs molecular iodine possibly generated iodine atom thus contributing toward free radical induced oxidative stress, whereas chitosan and Ag NPs facilitated the process of cell killing and thus collectively enhanced the potency of antimicrobial effect at the lowest concentrations of individual components.
Authors:
Madhuchanda Banerjee; Sadhucharan Mallick; Anumita Paul; Arun Chattopadhyay; Siddhartha Sankar Ghosh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  26     ISSN:  1520-5827     ISO Abbreviation:  Langmuir     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-13     Completed Date:  2010-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5901-8     Citation Subset:  IM    
Affiliation:
Centre for Nanotechnology, Indian Institute of Technology Guwahati, Guwahati-39, Assam, India.
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MeSH Terms
Descriptor/Qualifier:
Anti-Infective Agents / chemistry*,  pharmacology*
Chitosan / chemistry*
Escherichia coli / drug effects,  genetics,  metabolism
Green Fluorescent Proteins / genetics,  metabolism
Iodine / chemistry,  pharmacology*
Metal Nanoparticles / chemistry*
Microscopy, Electron, Transmission
Reactive Oxygen Species
Silver / chemistry*
Chemical
Reg. No./Substance:
0/Anti-Infective Agents; 0/Reactive Oxygen Species; 147336-22-9/Green Fluorescent Proteins; 7440-22-4/Silver; 7553-56-2/Iodine; 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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