Document Detail


The Hedgehog pathway promotes blood-brain barrier integrity and CNS immune quiescence.
MedLine Citation:
PMID:  22144466     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The blood-brain barrier (BBB) is composed of tightly bound endothelial cells (ECs) and perivascular astrocytes that regulate central nervous system (CNS) homeostasis. We showed that astrocytes secrete Sonic hedgehog and that BBB ECs express Hedgehog (Hh) receptors, which together promote BBB formation and integrity during embryonic development and adulthood. Using pharmacological inhibition and genetic inactivation of the Hh signaling pathway in ECs, we also demonstrated a critical role of the Hh pathway in promoting the immune quiescence of BBB ECs by decreasing the expression of proinflammatory mediators and the adhesion and migration of leukocytes, in vivo and in vitro. Overall, the Hh pathway provides a barrier-promoting effect and an endogenous anti-inflammatory balance to CNS-directed immune attacks, as occurs in multiple sclerosis.
Authors:
Jorge Ivan Alvarez; Aurore Dodelet-Devillers; Hania Kebir; Igal Ifergan; Pierre J Fabre; Simone Terouz; Mike Sabbagh; Karolina Wosik; Lyne Bourbonnière; Monique Bernard; Jack van Horssen; Helga E de Vries; Frédéric Charron; Alexandre Prat
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-12-01
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  334     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-23     Completed Date:  2012-01-25     Revised Date:  2012-02-01    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1727-31     Citation Subset:  IM    
Affiliation:
Neuroimmunology Unit, Center of Excellence in Neuromics, Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Faculty of Medicine, Université de Montréal, Montréal, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / metabolism*
Blood-Brain Barrier / cytology,  physiology*
Brain / immunology*,  physiology
CD4-Positive T-Lymphocytes / physiology
Cell Adhesion
Cell Movement
Cells, Cultured
Chemokines / metabolism
Electric Impedance
Encephalomyelitis, Autoimmune, Experimental / immunology,  metabolism
Endothelial Cells / metabolism*
Female
Hedgehog Proteins / genetics,  metabolism*
Humans
Inflammation Mediators / metabolism
Mice
Mice, Inbred C57BL
Multiple Sclerosis / immunology,  metabolism
Permeability
Signal Transduction*
Veratrum Alkaloids / pharmacology
Grant Support
ID/Acronym/Agency:
MOP74700//Canadian Institutes of Health Research; MOP81880//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Chemokines; 0/Hedgehog Proteins; 0/Inflammation Mediators; 0/SHH protein, human; 0/Shh protein, mouse; 0/Veratrum Alkaloids; 4449-51-8/cyclopamine
Comments/Corrections
Comment In:
Science. 2011 Dec 23;334(6063):1652-3   [PMID:  22194564 ]
Nat Rev Immunol. 2012 Jan;12(1):4   [PMID:  22193763 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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