Document Detail


Hebbian induction of LTP in visual cortex: perforated patch-clamp study in cultured neurons.
MedLine Citation:
PMID:  8747204     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. To see whether presynaptic activation paired with postsynaptic depolarization is necessary for the induction of long-term potentiation (LTP) in visual cortex or whether an activation of postsynaptic receptors in conjunction with depolarization is sufficient, we carried out perforated patch-clamp recordings with nystatin from cultured cortical neurons of rats. 2. Recorded neurons were monosynaptically activated either by electrical stimulation of an adjacent neuron or by direct activation of glutamate on "hot spots" of dendrites through iontophoresis or pressure ejection. In experiments in which cultured neurons were stained immunocytochemically with antibody against synaptophysin after electrophysiological recordings, hot spots were found to correspond to probable synaptic sites. 3. Excitatory postsynaptic currents (EPSCs) evoked by test stimulation applied to the adjacent neuron at 0.1 Hz were recorded at a holding potential of -60 or -70 mV for 5-10 min after an establishment of the whole cell recording configuration. Then, stimulation was paired with postsynaptic depolarization (0 mV for 200 ms) at 1 Hz for 30 or 60 s. LTP of EPSCs was induced in 7 of the 15 cells from which stable recordings were obtained for 18-30 min after pairing. 4. When postsynaptic depolarization was paired with direct glutamate application in the absence of presynaptic stimulation in 12 cells, only 1 showed LTP. Postsynaptic depolarization alone did not induce LTP in any of the six cells tested. Also, presynaptic stimulation alone did not induce LTP in any of the five cells tested. 5. These results suggest that the concurrent activation of presynaptic elements with postsynaptic depolarization is necessary for the induction of LTP in visual cortex.
Authors:
Y Otsu; F Kimura; T Tsumoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurophysiology     Volume:  74     ISSN:  0022-3077     ISO Abbreviation:  J. Neurophysiol.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-10-09     Completed Date:  1996-10-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375404     Medline TA:  J Neurophysiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2437-44     Citation Subset:  IM    
Affiliation:
Department of Neurophysiology, Osaka University Medical School, Suita, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Culture Techniques
Electric Stimulation
Electrophysiology
Female
Immunohistochemistry
Long-Term Potentiation / physiology*
Male
Neurons / physiology*
Patch-Clamp Techniques
Rats
Rats, Sprague-Dawley
Rats, Wistar
Receptors, Presynaptic / drug effects,  physiology
Visual Cortex / physiology*
Chemical
Reg. No./Substance:
0/Receptors, Presynaptic

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