Document Detail


Heavy metals induce phosphorylation of the Bcl-2 protein by Jun N-terminal kinase.
MedLine Citation:
PMID:  19007308     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Bcl-2 protein is one of the key components of biochemical pathways controlling programmed cell death. The function of this protein can be regulated by posttranslational modifications. Phosphorylation of Bcl-2 has been considered to be significantly associated with cell cycle arrest in the G2/M phase of the cell cycle, and with cell death caused by defects of microtubule dynamics. This study shows that phosphorylation of Bcl-2 can be induced by heavy metals due to activation of the Jun N-terminal kinase pathway that is not linked to the G2/M cell cycle arrest. Furthermore, we demonstrate that hyperphosphorylated Bcl-2 protein is a more potent inhibitor of zinc-induced cell death than its hypophosphorylated mutant form. These data suggest that regulation of Bcl-2 protein function by phosphorylation is an important part of cell responses to stress.
Authors:
Eva Ondrousková; Jana Slovácková; Vendula Pelková; Jirina Procházková; Karel Soucek; Petr Benes; Jan Smarda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological chemistry     Volume:  390     ISSN:  1431-6730     ISO Abbreviation:  Biol. Chem.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-17     Completed Date:  2009-03-12     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9700112     Medline TA:  Biol Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  49-58     Citation Subset:  IM    
Affiliation:
Institute of Experimental Biology, Faculty of Science, Masaryk University, Kotlárská 2, CZ-611 37 Brno, Czech Republic.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Cell Line, Tumor
Electrophoresis
Gene Expression Regulation, Neoplastic / drug effects
Humans
JNK Mitogen-Activated Protein Kinases / metabolism*
Metals, Heavy / pharmacology*
Phosphorylation / drug effects
Protein Processing, Post-Translational / drug effects
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Signal Transduction / drug effects
Stress, Physiological / drug effects
Zinc / pharmacology
Chemical
Reg. No./Substance:
0/Metals, Heavy; 0/Proto-Oncogene Proteins c-bcl-2; 7440-66-6/Zinc; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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