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Heating-induced bacteriological and biochemical modifications in human donor milk after Holder pasteurization.
MedLine Citation:
PMID:  21921811     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVES:: The objectives of this work were to enumerate and characterize the pathogenic potential of the Bacillus population that may survive Holder pasteurization of human milk and to evaluate the nutritional damage of this treatment using the furosine and lactulose indexes. MATERIALS AND METHODS:: Milk samples from 21 donors were heated at 62.5°C for 30 minutes. Bacterial counts, lactose, glucose, myo-inositol, lactulose and furosine were determined before and after the heat treatment. Some Bacillus cereus isolates that survived after pasteurization were evaluated for toxigenic potential. RESULTS:: Non-pasteurized milk samples showed bacterial growth in most of the agar media tested. Bacterial survival after pasteurization was only observed in three samples and, in these cases, the microorganisms isolated belonged to the species B. cereus. Furosine could not be detected in any of the samples while changes in lactose, glucose, and myo-inositol concentrations after Holder pasteurization were not relevant. Lactulose was below the detection limit of the analytical method in non pasteurized samples while it was found at low levels in 62% of the samples after Holder pasteurization. The lactation period influenced myo-inositol content since its concentration in transition milk was significantly higher than in mature or late lactation milk samples. CONCLUSIONS:: Holder pasteurization led to the destruction of bacteria present initially in donor milk samples, except some B. cereus that did not display a high virulence potential, and did not modify significantly the concentration of the compounds analyzed in this work.
Authors:
A Gómez de Segura; D Escuder; A Montilla; G Bustos; C Pallás; L Fernández; N Corzo; J M Rodríguez
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-14
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  -     ISSN:  1536-4801     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
*Dpto. Nutrición, Bromatología y Tecnología de los Alimentos. Universidad Complutense de Madrid, Spain †Servicio de Pediatría y Neonatología. Hospital Universitario 12 de Octubre, Madrid, Spain ‡Dpto. de Bioactividad y Análisis de Alimentos. Instituto de Investigación en Ciencias de la Alimentación, Madrid, Spain.
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