Document Detail


Heat treatment induces liver receptor homolog-1 expression in monkey and rat sertoli cells.
MedLine Citation:
PMID:  17170099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We demonstrated in this study that liver receptor homolog-1 (LRH-1) was expressed in the round spermatids in normal monkey testis, and no LRH-1 signal was observed in the Sertoli cells. After local warming (43 C) the monkey testis, however, LRH-1 expression was induced in the Sertoli cells in coincidence with activation of cytokeratin 18 (CK-18), a Sertoli cell dedifferentiated marker. Furthermore, we isolated rat primary Sertoli cells from testes at various stages of development and treated with 43 C water in vitro. The changes in LRH-1 as well as CK-18 expression were analyzed by confocal immunohistochemistry and Western blot. The results showed that LRH-1 was stage-dependently expressed in the Sertoli cells; no LRH-1-positive signal was detected in the cells obtained from the testes of adult rat on d 60 after birth when mature spermatozoa in the testis was completed. However, the mature Sertoli cells were warmed at the 43 C water bath for 15 min, and the LRH-1 signal was remarkably induced in a time-dependent manner, just like the changes of CK-18 expression in the Sertoli cells, suggesting that the heat-induced dedifferentiation of the mature Sertoli cells might be related to LRH-1 regulation. LRH-1 expression induced by the heat treatment was completely inhibited by the addition of ERK inhibitor U0126 in the culture, indicating that the heat-induced LRH-1 expression in the Sertoli cells may be regulated via ERK1/2 activation pathway. Testosterone was found to have no such effect on LRH-1 expression in the monkey and rat Sertoli cells.
Authors:
Jian Guo; Shi-Xin Tao; Min Chen; Yu-Qiang Shi; Zhu-Qiang Zhang; Yin-Chuan Li; Xue-Sen Zhang; Zhao-Yuan Hu; Yi-Xun Liu
Related Documents :
9038789 - Seasonal spermatogenesis and testosterone production in roe deer (capreolus capreolus).
2323999 - Preparation and short-term culture of enriched populations of guinea pig spermatocytes ...
19268429 - Deprival of testicular innervation induces apoptosis of leydig cells via caspase-8-depe...
6802089 - Stage dependent variation in mn2+-sensitive adenylyl cyclase (ac) activity in spermatid...
20403869 - Non-classical actions of testosterone and spermatogenesis.
6177569 - Stimulatory effect of lhrh and its agonists on leydig cell steroidogenesis in vitro.
55279 - Interference with proliferative activity in the cns and its relation to facial abnormal...
25216299 - Odour enrichment increases adult-born dopaminergic neurons in the mouse olfactory bulb.
21775119 - The effects of transformation and znt-1 silencing on zinc homeostasis in cultured cells.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-14
Journal Detail:
Title:  Endocrinology     Volume:  148     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-16     Completed Date:  2007-04-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1255-65     Citation Subset:  AIM; IM    
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25, Bei Si Huan Xi Lu, Beijing 100080, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Cryptorchidism / metabolism
DAX-1 Orphan Nuclear Receptor
DNA-Binding Proteins / metabolism
Dehydration / metabolism
Gene Expression Regulation* / drug effects
Hot Temperature*
Macaca fascicularis
Male
Rats
Receptors, Cytoplasmic and Nuclear / metabolism*
Receptors, Retinoic Acid / metabolism
Repressor Proteins / metabolism
Sertoli Cells / drug effects,  metabolism*
Testis / drug effects,  metabolism
Testosterone / pharmacology
Chemical
Reg. No./Substance:
0/DAX-1 Orphan Nuclear Receptor; 0/DNA-Binding Proteins; 0/NR5A2 protein, rat; 0/Nr0b1 protein, rat; 0/Receptors, Cytoplasmic and Nuclear; 0/Receptors, Retinoic Acid; 0/Repressor Proteins; 58-22-0/Testosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A topical parathyroid hormone/parathyroid hormone-related peptide receptor antagonist stimulates hai...
Next Document:  The orphan nuclear estrogen receptor-related receptor-alpha regulates cartilage formation in vitro: ...