Document Detail

Heat treatment induces liver receptor homolog-1 expression in monkey and rat sertoli cells.
MedLine Citation:
PMID:  17170099     Owner:  NLM     Status:  MEDLINE    
We demonstrated in this study that liver receptor homolog-1 (LRH-1) was expressed in the round spermatids in normal monkey testis, and no LRH-1 signal was observed in the Sertoli cells. After local warming (43 C) the monkey testis, however, LRH-1 expression was induced in the Sertoli cells in coincidence with activation of cytokeratin 18 (CK-18), a Sertoli cell dedifferentiated marker. Furthermore, we isolated rat primary Sertoli cells from testes at various stages of development and treated with 43 C water in vitro. The changes in LRH-1 as well as CK-18 expression were analyzed by confocal immunohistochemistry and Western blot. The results showed that LRH-1 was stage-dependently expressed in the Sertoli cells; no LRH-1-positive signal was detected in the cells obtained from the testes of adult rat on d 60 after birth when mature spermatozoa in the testis was completed. However, the mature Sertoli cells were warmed at the 43 C water bath for 15 min, and the LRH-1 signal was remarkably induced in a time-dependent manner, just like the changes of CK-18 expression in the Sertoli cells, suggesting that the heat-induced dedifferentiation of the mature Sertoli cells might be related to LRH-1 regulation. LRH-1 expression induced by the heat treatment was completely inhibited by the addition of ERK inhibitor U0126 in the culture, indicating that the heat-induced LRH-1 expression in the Sertoli cells may be regulated via ERK1/2 activation pathway. Testosterone was found to have no such effect on LRH-1 expression in the monkey and rat Sertoli cells.
Jian Guo; Shi-Xin Tao; Min Chen; Yu-Qiang Shi; Zhu-Qiang Zhang; Yin-Chuan Li; Xue-Sen Zhang; Zhao-Yuan Hu; Yi-Xun Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-14
Journal Detail:
Title:  Endocrinology     Volume:  148     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-16     Completed Date:  2007-04-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1255-65     Citation Subset:  AIM; IM    
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25, Bei Si Huan Xi Lu, Beijing 100080, China.
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MeSH Terms
Cells, Cultured
Cryptorchidism / metabolism
DAX-1 Orphan Nuclear Receptor
DNA-Binding Proteins / metabolism
Dehydration / metabolism
Gene Expression Regulation* / drug effects
Hot Temperature*
Macaca fascicularis
Receptors, Cytoplasmic and Nuclear / metabolism*
Receptors, Retinoic Acid / metabolism
Repressor Proteins / metabolism
Sertoli Cells / drug effects,  metabolism*
Testis / drug effects,  metabolism
Testosterone / pharmacology
Reg. No./Substance:
0/DAX-1 Orphan Nuclear Receptor; 0/DNA-Binding Proteins; 0/NR5A2 protein, rat; 0/Nr0b1 protein, rat; 0/Receptors, Cytoplasmic and Nuclear; 0/Receptors, Retinoic Acid; 0/Repressor Proteins; 58-22-0/Testosterone

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