| Heat shock treatment releases Penicillium phosphoglycopeptide. | |
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MedLine Citation:
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PMID: 9461674 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Filtrates of heat (54 degrees) treated day-5 Penicillium fellutanum cultures contained 70 mg of peptidophosphogalactomannan-II; an unheated control contained 30 mg. The polymer contained up to 60 phosphodiesters, and 5-O-beta-D-galactofuranosyl, mannopyranosyl, amino acyl and 2-aminoethanol residues. Its 13C NMR spectrum was nearly identical with that of the control polymer. The major 31P NMR signal was phosphocholine phosphodiester at delta 0.22 ppm: significant phosphodiester signals occurred at delta 1.15, 1.33 and 1.47. Dilute mineral acid released galactofuranosyl residues from the mannan. Signals at delta 1.15-1.47 ppm were associated with molecules of mass less than 3500 and contained galactose, 2-aminoethanol and peptide(s). After this acid treatment, signals at delta 1.0-0.22 remained associated with the mannan. Heat released up to 4-fold more of peptidophosphogalactomannan-III compared with the untreated control; carbohydrate and phosphate content, per mg polymer, were reduced by 4- and 2-fold, respectively. A galactofuranosyl-, phosphoryl- and amino acyl-containing polymer of Mr greater than 14,000 was solubilized by alkali treatment of P. fellutanum cell walls. |
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Authors:
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S J Bonetti; S E Groves; B Black; J E Gander |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Phytochemistry Volume: 47 ISSN: 0031-9422 ISO Abbreviation: Phytochemistry Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-03-03 Completed Date: 1998-03-03 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0151434 Medline TA: Phytochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 567-75 Citation Subset: B |
Affiliation:
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Department of Microbiology and Cell Science, University of Florida, Gainesville 32611-0700, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrate Sequence Cell Wall / chemistry Glycosaminoglycans / chemistry*, isolation & purification Hot Temperature Molecular Sequence Data Nuclear Magnetic Resonance, Biomolecular Penicillium / chemistry*, growth & development |
| Grant Support | |
ID/Acronym/Agency:
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GM 19978/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glycosaminoglycans; 52108-79-9/phosphogalactomannan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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