Document Detail

Heat shock proteins form part of a danger signal cascade in response to lipopolysaccharide and GroEL.
MedLine Citation:
PMID:  16792689     Owner:  NLM     Status:  MEDLINE    
An increasing number of cell types, including peripheral blood mononuclear cells (PBMCs), have been demonstrated to release heat shock proteins (Hsps). In this paper we investigate further the hypothesis that Hsps are danger signals. PBMCs and Jurkat cells released Hsp70 (0.22 and 0.7 ng/10(6) cells, respectively) into medium over 24 h at 37 degrees C. Release of Hsp70 was stimulated 10-fold by GroEL (P < 0.001) and more than threefold by lipopolysaccharide (LPS) (P < 0.001). Although Hsp60 could be detected in the medium of cells cultured at 37 degrees C for 24 h, the low rates of release were due probably to cell damage. Significant release of Hsp60 was observed when Jurkat cells were exposed to GroEL (2.88 ng/10(6) cells) or LPS (1.40 ng/10(6) cells). The data are consistent with the hypothesis that Hsp70 and Hsp60 are part of a danger signalling cascade in response to bacterial infection.
E L Davies; M M F V G Bacelar; M J Marshall; E Johnson; T D Wardle; S M Andrew; J H H Williams
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  145     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-23     Completed Date:  2006-08-07     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  183-9     Citation Subset:  IM    
Chester Centre for Stress Research, Biological Sciences, University of Chester, Chester, UK.
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MeSH Terms
Antigens, Bacterial / pharmacology*
Blotting, Western / methods
Cell Line
Cells, Cultured
Chaperonin 60 / analysis,  pharmacology,  secretion
HSP70 Heat-Shock Proteins / analysis,  secretion
Heat-Shock Proteins / analysis,  secretion*
Jurkat Cells
L-Lactate Dehydrogenase / analysis
Leukocytes, Mononuclear / drug effects,  secretion*
Lipopolysaccharides / pharmacology
Signal Transduction / drug effects*
Stromal Cells / drug effects,  secretion
T-Lymphocytes / drug effects,  secretion
Time Factors
Reg. No./Substance:
0/Antigens, Bacterial; 0/Chaperonin 60; 0/HSP70 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/Lipopolysaccharides; EC Dehydrogenase

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