Document Detail

Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats.
MedLine Citation:
PMID:  22821472     Owner:  NLM     Status:  MEDLINE    
We evaluated associations between the concentrations of heat shock proteins (hsp60 and hsp70) and their respective antibodies, alterations in maternal reproductive performance, and fetal malformations in pregnant rats with hyperglycemia. Mild diabetes (MD) or severe diabetes (SD) was induced in Sprague-Dawley rats prior to mating; non-treated non-diabetic rats (ND) served as controls. On day 21 of pregnancy, maternal blood was analyzed for hsp60 and hsp70 and their antibodies; and fetuses were weighed and analyzed for congenital malformations. Hsp and anti-hsp levels were correlated with blood glucose levels during gestation. There was a positive correlation between hsp60 and hsp70 levels and the total number of malformations (R = 0.5908, P = 0.0024; R = 0.4877, P = 0.0134, respectively) and the number of malformations per fetus (R = 0.6103, P = 0.0015; R = 0.4875, P = 0.0134, respectively). The anti-hsp60 IgG concentration was correlated with the number of malformations per fetus (R = 0.3887, P = 0.0451) and the anti-hsp70 IgG level correlated with the total number of malformations (R = 0.3999, P = 0.0387). Moreover, both hsp and anti-hsp antibodies showed negative correlations with fetal weight. The results suggest that there is a relationship between hsp60 and hsp70 levels and their respective antibodies and alterations in maternal reproductive performance and impaired fetal development and growth in pregnancies associated with diabetes.
Felipe Hiroshi Saito; Débora Cristina Damasceno; Bruna Dallaqua; Iara Moreno Linhares; Marilza Vieira Cunha Rudge; Iracema De Mattos Paranhos Calderon; Steven S Witkin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-23
Journal Detail:
Title:  Cell stress & chaperones     Volume:  18     ISSN:  1466-1268     ISO Abbreviation:  Cell Stress Chaperones     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-11-27     Completed Date:  2013-05-09     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  9610925     Medline TA:  Cell Stress Chaperones     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  25-33     Citation Subset:  IM    
Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP-Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil.
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MeSH Terms
Antibodies / blood,  immunology
Blood Glucose / analysis
Chaperonin 60 / immunology,  metabolism*
Diabetes Mellitus, Experimental / immunology,  metabolism*,  pathology
Fetal Development
HSP70 Heat-Shock Proteins / immunology,  metabolism*
Pregnancy, Animal*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Antibodies; 0/Blood Glucose; 0/Chaperonin 60; 0/HSP70 Heat-Shock Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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