| Heat shock protein 72 and apoptosis indicate cardiac decompensation during early multiple organ failure in sheep. | |
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MedLine Citation:
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PMID: 14985962 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Inducible heat shock protein 72 (HSP 72) preserves myocardial function and prevents apoptosis. We investigated the expression and localization of HSP 72 and apoptosis in our previously described new model of multiple organ failure. DESIGN: Eighteen adult-instrumented sheep and three healthy controls were randomly assigned to one of three groups: (a) norfenefrine-masked hypovolemia plus endotoxemia (NMH+ENDO); (b) norfenefrine-masked hypovolemia without endotoxemia (NMH); (c) recurrent endotoxemia during normovolemia (ENDO); and (d) normovolemia without endotoxemia (CONTROLS). MEASUREMENTS AND RESULTS: Hearts were analyzed by light microscopy, Western blots, immunohistochemistry, and TUNEL staining. HSP 72 expression was approximately threefold increased in NMH+ENDO compared with the other groups ( p<0.05) and was localized mainly in left ventricular cardiomyocytes. HSP 72 was elevated in animals with norfenefrine-refractory shock compared to survivors ( p=0.015). TUNEL-positive cells in the left ventricle were significantly elevated in the NMH+ENDO group ( p=0.05) and correlated with HSP 72 expression (r=0.51, p=0.018). HSP 72 correlated positively with heart rate (r=0.76, p<0.0001), the prefinal hourly dose of norfenefrine (r=0.88, p<0.0001), and negatively with left ventricular stroke work index (r=-0.52, p=0.028). Double staining revealed TUNEL-positive cells with and without HSP 72 expression. Micronecroses were only detectable in NMH and NMH+ENDO without intergroup difference or correlations with hemodynamics. CONCLUSION: HSP 72 overexpression and apoptosis, but not necrosis, indicate cardiovascular decompensation and poor outcome during early multiple organ failure. |
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Authors:
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Hideo A Baba; Jeremias Wohlschlaeger; Henning D Stubbe; Florian Grabellus; Hugo Van Aken; Klaus J Schmitz; Friedrich Otterbach; Kurt W Schmid; Christian August; Bodo Levkau; Frank Hinder |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2004-02-24 |
Journal Detail:
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Title: Intensive care medicine Volume: 30 ISSN: 0342-4642 ISO Abbreviation: Intensive Care Med Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-06-28 Completed Date: 2005-01-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7704851 Medline TA: Intensive Care Med Country: United States |
Other Details:
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Languages: eng Pagination: 1405-13 Citation Subset: IM |
Affiliation:
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Institut für Pathologie, Universität Essen, Hufelandstrasse 55, 45147 Essen, Germany. hideo.baba@medizin.uni-essen.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* Blotting, Western Disease Models, Animal HSP72 Heat-Shock Proteins Heat-Shock Proteins / analysis*, metabolism Immunohistochemistry In Situ Nick-End Labeling Multiple Organ Failure / metabolism, pathology* Myocardium / chemistry, metabolism, pathology Random Allocation Sheep |
| Chemical | |
Reg. No./Substance:
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0/HSP72 Heat-Shock Proteins; 0/Heat-Shock Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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