Document Detail

Heat-shock protein 25 ameliorates calcium oxalate crystal-mediated oxidative stress in renal epithelial cells.
MedLine Citation:
PMID:  17034610     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate whether the antioxidant protection attributed to small heat-shock proteins (sHSPs) affects calcium oxalate stone formation, a pro-oxidant disease. MATERIALS AND METHODS: Canine distal tubular epithelial cells (Madin-Darby canine kidney, MDCK cells) were grown as confluent monolayers. Treatment regimens included control and HS-treated cells (37 degrees C and 42 degrees C for 1 h) with or without calcium oxalate monohydrate (COM) or free oxalate treatment (28 microg/cm2) 16 h later. In digitonin-permeabilized cells, O2- was measured by lucigenin-enhanced chemiluminescence over a 5-min period, to measure mitochondrial O2- production. Protein expression was assessed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis Western blot analysis using specific antibodies. RESULTS: COM significantly increased O2- production in MDCK cells. HS treatment, which up-regulated HSP25 expression, significantly decreased this O2- production (P < 0.05) but had no effect in control cells. In COM-treated cells (20 h) there was a marked and significant down-regulation of both HSP 25, HSP 70 and heme oxygenase-1 expression compared to cells treated with HS alone (P < 0.05). Free oxalate had no effect on HSP 25 expression. CONCLUSIONS: The results suggest that the COM-induced increase in mitochondrial O2- production in MDCK cells is ameliorated by HSP 25 up-regulation via HS. Specific COM inhibition of HSP 25, HSP 70 and heme oxygenase-1 up-regulation suggests that COM-induced reactive oxygen species damage is unable to benefit from HSP-associated physiological resistance.
Amit B Patel; William G Robertson; Simon Choong; John S Hothersall
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  BJU international     Volume:  98     ISSN:  1464-4096     ISO Abbreviation:  BJU Int.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-12     Completed Date:  2007-01-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100886721     Medline TA:  BJU Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  1094-9     Citation Subset:  IM    
Centre for Treatment and Prevention of Kidney Stone disease, Institute of Urology, University College London, UK.
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MeSH Terms
Blotting, Western
Calcium Oxalate / pharmacology*
Epithelial Cells / drug effects
HSP27 Heat-Shock Proteins
Heat-Shock Proteins / metabolism*
Heat-Shock Proteins, Small / pharmacology*
Kidney Calculi / prevention & control*
Neoplasm Proteins / metabolism*
Oxidative Stress / drug effects*
Reg. No./Substance:
0/HSP27 Heat-Shock Proteins; 0/HSPB1 protein, human; 0/Heat-Shock Proteins; 0/Heat-Shock Proteins, Small; 0/Neoplasm Proteins; 25454-23-3/Calcium Oxalate

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