| Heat shock-induced enhancement of osteoblastic differentiation of hTERT-immortalized mesenchymal stem cells. | |
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MedLine Citation:
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PMID: 16804024 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Heat shock (HS)-induced stress response in human cells results in a variety of biological effects and is known to induce the transcription of heat-shock proteins, which help the cells to cope with different kinds of stress. We have studied the effects of HS on the differentiation of human mesenchymal stem cells (hMSCs) into osteoblastic cells. As a model for hMSCs we used a telomerase-immortalized hMSC line designated hMSC-TERT. Cells were exposed to 1 h HS at 41 degrees C, 42.5 degrees C, or 44 degrees C prior to incubation in a medium containing either 10(-8) M 1alpha,25-dihydroxy-vitamin-D(3) (calcitriol) or 10(-8) M calcitriol, 50 microg/mL L-ascorbic acid, and 10 mM beta-glycerophosphate followed by an analysis of induction of osteoblast differentiation and the formation of mineralized matrix, respectively. Our results indicate that the exposure of cells to mild heat stress enhances the extent of differentiation of hMSCs by 12% to 42%. These effects are an expression of the phenomenon of mild stress-induced hormesis. |
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Authors:
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Rune Nørgaard; Moustapha Kassem; Suresh I S Rattan |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Annals of the New York Academy of Sciences Volume: 1067 ISSN: 0077-8923 ISO Abbreviation: Ann. N. Y. Acad. Sci. Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-06-28 Completed Date: 2006-07-18 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7506858 Medline TA: Ann N Y Acad Sci Country: United States |
Other Details:
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Languages: eng Pagination: 443-7 Citation Subset: IM |
Affiliation:
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Laboratory of Cellular Ageing, Danish Centre for Molecular Gerontology, Department of Molecular Biology, University of Aarhus, Denmark. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Ascorbic Acid / pharmacology Bone Marrow Cells / cytology Calcitriol / pharmacology Cell Differentiation / drug effects* Cell Line, Transformed Culture Media / chemistry, pharmacology DNA-Binding Proteins / metabolism* Dose-Response Relationship, Drug Female Glycerophosphates / pharmacology Heat-Shock Response / drug effects* Hot Temperature Humans Ilium / cytology Male Mesenchymal Stem Cells / cytology*, metabolism Osteoblasts / cytology, drug effects*, enzymology*, metabolism Telomerase / metabolism* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/DNA-Binding Proteins; 0/Glycerophosphates; 17181-54-3/beta-glycerophosphoric acid; 32222-06-3/Calcitriol; 50-81-7/Ascorbic Acid; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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