Document Detail

Heat shock-induced enhancement of osteoblastic differentiation of hTERT-immortalized mesenchymal stem cells.
MedLine Citation:
PMID:  16804024     Owner:  NLM     Status:  MEDLINE    
Heat shock (HS)-induced stress response in human cells results in a variety of biological effects and is known to induce the transcription of heat-shock proteins, which help the cells to cope with different kinds of stress. We have studied the effects of HS on the differentiation of human mesenchymal stem cells (hMSCs) into osteoblastic cells. As a model for hMSCs we used a telomerase-immortalized hMSC line designated hMSC-TERT. Cells were exposed to 1 h HS at 41 degrees C, 42.5 degrees C, or 44 degrees C prior to incubation in a medium containing either 10(-8) M 1alpha,25-dihydroxy-vitamin-D(3) (calcitriol) or 10(-8) M calcitriol, 50 microg/mL L-ascorbic acid, and 10 mM beta-glycerophosphate followed by an analysis of induction of osteoblast differentiation and the formation of mineralized matrix, respectively. Our results indicate that the exposure of cells to mild heat stress enhances the extent of differentiation of hMSCs by 12% to 42%. These effects are an expression of the phenomenon of mild stress-induced hormesis.
Rune Nørgaard; Moustapha Kassem; Suresh I S Rattan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1067     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-06-28     Completed Date:  2006-07-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  443-7     Citation Subset:  IM    
Laboratory of Cellular Ageing, Danish Centre for Molecular Gerontology, Department of Molecular Biology, University of Aarhus, Denmark.
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MeSH Terms
Ascorbic Acid / pharmacology
Bone Marrow Cells / cytology
Calcitriol / pharmacology
Cell Differentiation / drug effects*
Cell Line, Transformed
Culture Media / chemistry,  pharmacology
DNA-Binding Proteins / metabolism*
Dose-Response Relationship, Drug
Glycerophosphates / pharmacology
Heat-Shock Response / drug effects*
Hot Temperature
Ilium / cytology
Mesenchymal Stem Cells / cytology*,  metabolism
Osteoblasts / cytology,  drug effects*,  enzymology*,  metabolism
Telomerase / metabolism*
Time Factors
Reg. No./Substance:
0/Culture Media; 0/DNA-Binding Proteins; 0/Glycerophosphates; 17181-54-3/beta-glycerophosphoric acid; 32222-06-3/Calcitriol; 50-81-7/Ascorbic Acid; EC protein, human; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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