Document Detail

Heat-shock-induced cellular responses to temperature elevations occurring during orthopaedic cutting.
MedLine Citation:
PMID:  22915633     Owner:  NLM     Status:  MEDLINE    
Severe heat-shock to bone cells caused during orthopaedic procedures can result in thermal damage, leading to cell death and initiating bone resorption. By contrast, mild heat-shock has been proposed to induce bone regeneration. In this study, bone cells are exposed to heat-shock for short durations occurring during surgical cutting. Cellular viability, necrosis and apoptosis are investigated immediately after heat-shock and following recovery of 12, 24 h and 4 days, in osteocyte-like MLO-Y4 and osteoblast-like MC3T3-E1 cells, using flow cytometry. The regeneration capacity of heat-shocked Balb/c mesenchymal stem cells (MSCs) and MC3T3-E1s has been investigated following 7 and 14 day's recovery, by quantifying proliferation, differentiation and mineralization. An immediate necrotic response to heat-shock was shown in cells exposed to elevated temperatures (45°C, 47°C and most severe at 60°C). A longer-term apoptotic response is induced in MLO-Y4s and, to a lesser extent, in MC3T3-E1s. Heat-shock-induced differentiation and mineralization by MSCs. These findings indicate that heat-shock is more likely to induce apoptosis in osteocytes than osteoblasts, which might reflect their role as sensors detecting and communicating damage within bone. Furthermore, it is shown for the first time that mild heat-shock (less than equal to 47°C) for durations occurring during surgical cutting can positively enhance osseointegration by osteoprogenitors.
E B Dolan; M G Haugh; D Tallon; C Casey; L M McNamara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-22
Journal Detail:
Title:  Journal of the Royal Society, Interface / the Royal Society     Volume:  9     ISSN:  1742-5662     ISO Abbreviation:  J R Soc Interface     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-10-26     Completed Date:  2013-06-19     Revised Date:  2013-12-12    
Medline Journal Info:
Nlm Unique ID:  101217269     Medline TA:  J R Soc Interface     Country:  England    
Other Details:
Languages:  eng     Pagination:  3503-13     Citation Subset:  IM    
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MeSH Terms
3T3 Cells
Bone Resorption
Calcification, Physiologic
Calcium / metabolism
Cell Differentiation
Cell Line
Cell Proliferation
Heat-Shock Response*
Mesenchymal Stromal Cells / physiology*
Mice, Inbred BALB C
Orthopedic Procedures / adverse effects*
Osteoblasts / cytology,  physiology
Osteocytes / cytology,  physiology
Time Factors
Reg. No./Substance:

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