| Heat-regulated expression of the hepatitis B virus surface antigen in the human Wish cell line. | |
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MedLine Citation:
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PMID: 3660944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The DNA fragment coding for the hepatitis B virus surface antigen (HBsAg) was placed under the control of a human 70 kDa heat-shock protein (hsp70) promotor sequence. This plasmid construct has been used in transfection experiments to establish a stable amnion cell line of human origin (Wish), expressing an HBsAg in a heat-regulated fashion. Post-translational modifications, such as assembly, glycosylation, secretion and production of both major and middle S proteins appear to function normally. In addition, production of HBsAg under various protocols of heat induction is described. After inoculation into nude mice, development of tumours has been observed at the site of injection. Tumour cells, dispersed by means of collagenase or trypsin treatment from excised tumours, and subsequently seeded into Petri dishes, were able to secrete the same quantities of HBsAg after heat induction as were cells of the original cell line. |
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Authors:
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M Dreano; X Fouillet; J Brochot; J M Vallet; M L Michel; D Rungger; P Bromley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Virus research Volume: 8 ISSN: 0168-1702 ISO Abbreviation: Virus Res. Publication Date: 1987 Jul |
Date Detail:
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Created Date: 1987-11-05 Completed Date: 1987-11-05 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8410979 Medline TA: Virus Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 43-59 Citation Subset: IM |
Affiliation:
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Battelle Research Center, Geneva, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line DNA, Recombinant Gene Expression Regulation Heat-Shock Proteins / genetics* Hepatitis B Surface Antigens / genetics* Hot Temperature Humans Mice Mice, Nude Neoplasms, Experimental / pathology, physiopathology Polymers Promoter Regions, Genetic* Protein Binding Serum Albumin / metabolism Transfection |
| Chemical | |
Reg. No./Substance:
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0/DNA, Recombinant; 0/Heat-Shock Proteins; 0/Hepatitis B Surface Antigens; 0/Polymers; 0/Serum Albumin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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