Document Detail

Heat-regulated expression of the hepatitis B virus surface antigen in the human Wish cell line.
MedLine Citation:
PMID:  3660944     Owner:  NLM     Status:  MEDLINE    
The DNA fragment coding for the hepatitis B virus surface antigen (HBsAg) was placed under the control of a human 70 kDa heat-shock protein (hsp70) promotor sequence. This plasmid construct has been used in transfection experiments to establish a stable amnion cell line of human origin (Wish), expressing an HBsAg in a heat-regulated fashion. Post-translational modifications, such as assembly, glycosylation, secretion and production of both major and middle S proteins appear to function normally. In addition, production of HBsAg under various protocols of heat induction is described. After inoculation into nude mice, development of tumours has been observed at the site of injection. Tumour cells, dispersed by means of collagenase or trypsin treatment from excised tumours, and subsequently seeded into Petri dishes, were able to secrete the same quantities of HBsAg after heat induction as were cells of the original cell line.
M Dreano; X Fouillet; J Brochot; J M Vallet; M L Michel; D Rungger; P Bromley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virus research     Volume:  8     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1987 Jul 
Date Detail:
Created Date:  1987-11-05     Completed Date:  1987-11-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  43-59     Citation Subset:  IM    
Battelle Research Center, Geneva, Switzerland.
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MeSH Terms
Cell Line
DNA, Recombinant
Gene Expression Regulation
Heat-Shock Proteins / genetics*
Hepatitis B Surface Antigens / genetics*
Hot Temperature
Mice, Nude
Neoplasms, Experimental / pathology,  physiopathology
Promoter Regions, Genetic*
Protein Binding
Serum Albumin / metabolism
Reg. No./Substance:
0/DNA, Recombinant; 0/Heat-Shock Proteins; 0/Hepatitis B Surface Antigens; 0/Polymers; 0/Serum Albumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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