Document Detail


Heat-induced phosphorylation of NBS1 in human skin fibroblast cells.
MedLine Citation:
PMID:  16823774     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
NBS1 is known to be involved in DNA damage-induced cellular responses after exposure to ionizing radiation (IR). Phosphorylation of NBS1 contributes to cell-cycle checkpoints. The aim of this study was to determine whether heat exposure induces or stimulates cellular responses mediated by the phosphorylation of NBS1 in human skin fibroblast cell lines. The results of immunofluorescent staining and Western blot analysis showed that NBS1 proteins are phosphorylated after exposure to heat in the nucleus of a normal skin fibroblast cell line (82-6 cells). This suggests that the NBS1-mediated signal transduction could be induced by heat. We further examined whether a deficiency in the NBS1 protein modifies heat sensitivity in human skin fibroblast cell lines. A skin fibroblast cell line (Gmtert), derived from a Nijmegen breakage syndrome (NBS) patient containing mutant NBS1, showed higher sensitivity to heat than the same cell line transfected with the wild-type copy of the NBS1 gene. We also showed that transfection of a DNA cassette expressing small interference RNA (siRNA) targeted to NBS1 into 82-6 cells enhanced cell sensitivity to heat. These results suggest that NBS1 is involved in cellular responses to DNA damage which is induced by heat exposure as well as by radiation exposure in human skin fibroblast cells.
Authors:
Ken Ohnishi; Zorica Scuric; David Yau; Robert H Schiestl; Noritomo Okamoto; Akihisa Takahashi; Takeo Ohnishi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  99     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-27     Completed Date:  2007-01-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1642-50     Citation Subset:  IM    
Copyright Information:
(c) 2006 Wiley-Liss, Inc.
Affiliation:
Department of Biology, Nara Medical University School of Medicine, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Blotting, Western
Cell Cycle Proteins / metabolism*
DNA Primers
Fibroblasts / metabolism
Fluorescent Antibody Technique
Hot Temperature*
Humans
Nuclear Proteins / metabolism*
Phosphorylation
Protein Transport
Skin / cytology,  metabolism*
Grant Support
ID/Acronym/Agency:
R01 ES 09519/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA Primers; 0/NBN protein, human; 0/Nuclear Proteins

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