| Heat-induced modulation of lamin B content in two different cell lines. | |
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MedLine Citation:
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PMID: 10572245 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous work in our laboratory indicates that the nuclear matrix protein lamin B is a "prompt" heat shock protein, which increases significantly when human U-1 melanoma and HeLa cells are exposed to 45.5 degrees C for 5-40 min. Using Western blotting, we found that the lamin B content in U-1 and HeLa cells increased to a greater extent during post-heat incubation at 37 degrees C than during the heat dose itself. When HeLa cells were heated at 45.5 degrees C for 30 min, and then incubated at 37 degrees C for up to 7 h, lamin B content was increased significantly (1.69-fold maximum increase at 3 h) compared to unincubated heated cells. Also, thermotolerant HeLa cells showed a greater increase (up to 1.72-fold) in lamin B content during subsequent heating compared to nontolerant cells. The increase in lamin B content in thermotolerant cells, or when heated cells were incubated at 37 degrees C, was also observed in U-1 cells. HeLa cells heated in the presence of glycerol (a heat protector) showed a 1.21-1.72-fold increase in lamin B content compared to cells heated for 10-30 min without glycerol. In contrast, lamin B content decreased 1.23-1.85-fold when cells were heated for 10-30 min in the presence of procaine (a heat sensitizer) compared to cells heated without procaine. These data suggest that lamin B may play an important role in the heat shock response, and that modulation of lamin B content by heat sensitizers or protectors may play a role in regulation of heat sensitivity. |
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Authors:
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W G Zhu; Z V Roberts; J R Dynlacht |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 75 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 1999 Dec |
Date Detail:
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Created Date: 2000-01-14 Completed Date: 2000-01-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 620-8 Citation Subset: IM |
Affiliation:
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Indiana University School of Medicine, Department of Radiation Oncology, Indiana Cancer Pavilion, Indianapolis, Indiana 46202, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anesthetics, Local
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pharmacology Blotting, Western Cell Survival / drug effects Glycerol / pharmacology Heat-Shock Response / drug effects Hela Cells / drug effects, metabolism* Humans Lamin Type B Lamins Melanoma / metabolism* Nuclear Proteins / metabolism* Procaine / pharmacology Time Factors Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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CA64674/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anesthetics, Local; 0/Lamin Type B; 0/Lamins; 0/Nuclear Proteins; 56-81-5/Glycerol; 59-46-1/Procaine |
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