Document Detail

Heat denaturation of serum albumin monitored by 1-anilino-naphthalene-8-sulfonate.
MedLine Citation:
PMID:  7378445     Owner:  NLM     Status:  MEDLINE    
The process of heat denaturation of serum albumin, and the properties of several denatured components of albumin were studied using 1-anilino-naphthalene-8-sulfonate as a probe dye. Like native albumin, these protein species all induce fluorescence of the dye with maximum emission at 470 nm when excited at 380 nm. However, the affinity of albumin for the dye decreased on denaturation. This fluorescent dye bound competitively to both native and denatured albumin with another probe dye, 2-(4'-hydroxyphenylazo)benzoic acid, has a specific absorption band at about 480 nm on binding with native albumin. Fatty acids, such as lauric acid, inhibited the interaction of 1-anilinonaphthalene-8-sulfonate with native albumin, but had little effect on its binding with denatured albumin.
H Terada; K Hiramatsu; K Aoki
Related Documents :
6421865 - A competitive ligand binding assay for measurement of thyroid hormone-binding inhibitor...
7150265 - The effect of beta-adrenergic agonists on the membrane potential of fat-cell mitochondr...
369855 - Valproic acid binding to human serum albumin and determination of free fraction in the ...
10441365 - Influence of protein surface morphology on the ultrafiltration flux resistance of bovin...
4146575 - Inhibition by gamma-hydroxybutyrate of chlorpromazine-induced increase in homovanillic ...
15330635 - Alkoxy-, acyloxy-, and bromomethylation of resorcinarenes.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  622     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1980 Apr 
Date Detail:
Created Date:  1980-08-28     Completed Date:  1980-08-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  161-70     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Anilino Naphthalenesulfonates*
Hot Temperature
Protein Binding
Protein Conformation
Protein Denaturation
Serum Albumin, Bovine*
Spectrometry, Fluorescence
Reg. No./Substance:
0/Anilino Naphthalenesulfonates; 0/Serum Albumin, Bovine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Activity of heparin-releasable liver lipase. Dependence on the degree of saturation of the fatty aci...
Next Document:  Marine biopolymers with cell specificity. II. Purification and characterization of agglutinins from ...