Document Detail


Heart-type fatty acid-binding protein reciprocally regulates glucose and fatty acid utilization during exercise.
MedLine Citation:
PMID:  15454399     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of heart-type cytosolic fatty acid-binding protein (H-FABP) in mediating whole body and muscle-specific long-chain fatty acid (LCFA) and glucose utilization was examined using exercise as a phenotyping tool. Catheters were chronically implanted in a carotid artery and jugular vein of wild-type (WT, n = 8), heterozygous (H-FABP(+/-), n = 8), and null (H-FABP(-/-), n = 7) chow-fed C57BL/6J mice, and mice were allowed to recover for 7 days. After a 5-h fast, conscious, unrestrained mice were studied during 30 min of treadmill exercise (0.6 mph). A bolus of [(125)I]-15-(p-iodophenyl)-3-R,S-methylpentadecanoic acid and 2-deoxy-[(3)H]glucose was administered to obtain rates of whole body metabolic clearance (MCR) and indexes of muscle LCFA (R(f)) and glucose (R(g)) utilization. Fasting, nonesterified fatty acids (mM) were elevated in H-FABP(-/-) mice (2.2 +/- 0.9 vs. 1.3 +/- 0.1 and 1.3 +/- 0.2 for WT and H-FABP(+/-)). During exercise, blood glucose (mM) increased in WT (11.7 +/- 0.8) and H-FABP(+/-) (12.6 +/- 0.9) mice, whereas H-FABP(-/-) mice developed overt hypoglycemia (4.8 +/- 0.8). Examination of tissue-specific and whole body glucose and LCFA utilization demonstrated a dependency on H-FABP with exercise in all tissues examined. Reductions in H-FABP led to decreasing exercise-stimulated R(f) and increasing R(g) with the most pronounced effects in heart and soleus muscle. Similar results were seen for MCR with decreasing LCFA and increasing glucose clearance with declining levels of H-FABP. These results show that, in vivo, H-FABP has reciprocal effects on glucose and LCFA utilization and whole body fuel homeostasis when metabolic demands are elevated by exercise.
Authors:
Jane Shearer; Patrick T Fueger; Jeffrey N Rottman; Deanna P Bracy; Bert Binas; David H Wasserman
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2004-09-28
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  288     ISSN:  0193-1849     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-07     Completed Date:  2005-02-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E292-7     Citation Subset:  IM    
Affiliation:
Dept. of Molecular Physiology and Biophysics, Vanderbilt University, 823 Light Hall, Nashville, TN 37232-0615, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Glucose / metabolism*
Carrier Proteins / metabolism*
Fatty Acid-Binding Proteins
Fatty Acids / blood*
Homeostasis / physiology
Metabolic Clearance Rate
Mice
Mice, Inbred C57BL
Mice, Knockout
Muscle, Skeletal / physiology*
Physical Conditioning, Animal / physiology*
Physical Exertion / physiology*
Grant Support
ID/Acronym/Agency:
DK 54902/DK/NIDDK NIH HHS; U24 DK 59637/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Carrier Proteins; 0/Fatty Acid-Binding Proteins; 0/Fatty Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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