Document Detail


Healing Characteristics of Electrospun, Polyurethane Grafts with Different Porosity.
MedLine Citation:
PMID:  23237988     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Pore size and porosity control rate and depth of cellular migration in electrospun vascular fabrics and thereby have a strong impact on long-term graft success. In this study we investigated the effect of graft porosity on cell migration in-vitro and in-vivo. Polyetherurethane (PU) grafts were fabricated by electrospinning as fine mesh, low porosity grafts [void fraction (VF): 53%] and coarse mesh, high porosity grafts [VF: 80%]. The fabricated grafts were evaluated in-vitro for endothelial cell attachment and proliferation. Prostheses were investigated in a rat model for either 7 days, 1, 3 or 6 months (n=7 per time point) and analyzed after retrieval by biomechanical analysis and various histological techniques. Cell migration was calculated by computer-assisted morphometry. In vitro, fine pore mesh favored early cell attachment. In-vivo, coarse mesh grafts revealed significantly higher cell populations at all time points in all areas of the conduit wall. Biomechanical tests indicated sufficient compliance, tensile and suture retention strength before and after implantation. Increased porosity improves host cell ingrowth and survival in electrospun conduits. These conduits show successful natural host vessel reconstitution without limitation of biomechanical properties.
Authors:
Helga Bergmeister; Catharina Schreiber; Christian Grasl; Ingrid Walter; Roberto Plasenzotti; Martin Stoiber; David Bernhard; Heinrich Schima
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-10
Journal Detail:
Title:  Acta biomaterialia     Volume:  -     ISSN:  1878-7568     ISO Abbreviation:  Acta Biomater     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ltd.
Affiliation:
Division of Biomedical Research, Medical University Vienna, Austria; Ludwig Boltzmann Cluster for Cardiovascular Research, Vienna, Austria. Electronic address: helga.bergmeister@meduniwien.ac.at.
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