| Headspace solid-phase microextraction with 1-pyrenyldiazomethane in-fibre derivatisation for analysis of faecal short-chain fatty acids. | |
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MedLine Citation:
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PMID: 10437678 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Short chain fatty acids (SFCAs) are nutritionally important products of colonic bacteria. Their analysis in faeces is problematic. We report a headspace solid-phase microextraction procedure in which faecal SCFAs are derivatised on the fibre in-situ with 1-pyrenyldiazomethane. With this method sharp, well-resolved chromatographic peaks were obtained with no interferences. Inclusion of deuterated analogues enabled accurate quantification. Good linearity, recoveries and precision were achieved. Differences observed between the SCFA profiles of normal subjects and patients with cystic fibrosis indicate the potential of this new technique for clinical studies. 2-Methylbutyric acid was found in all faecal samples. Few have reported this before. |
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Authors:
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G A Mills; V Walker; H Mughal |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical sciences and applications Volume: 730 ISSN: 1387-2273 ISO Abbreviation: J. Chromatogr. B Biomed. Sci. Appl. Publication Date: 1999 Jun |
Date Detail:
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Created Date: 1999-09-10 Completed Date: 1999-09-10 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9714109 Medline TA: J Chromatogr B Biomed Sci Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 113-22 Citation Subset: IM |
Affiliation:
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School of Pharmacy and Biomedical Sciences, University of Portsmouth, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Case-Control Studies Cystic Fibrosis / metabolism Fatty Acids / analysis* Feces / chemistry* Humans Indicators and Reagents / chemistry Pyrenes / chemistry Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Indicators and Reagents; 0/Pyrenes; 78377-23-8/1-pyrenyldiazomethane |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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