Document Detail

Headspace solid-phase microextraction with 1-pyrenyldiazomethane in-fibre derivatisation for analysis of faecal short-chain fatty acids.
MedLine Citation:
PMID:  10437678     Owner:  NLM     Status:  MEDLINE    
Short chain fatty acids (SFCAs) are nutritionally important products of colonic bacteria. Their analysis in faeces is problematic. We report a headspace solid-phase microextraction procedure in which faecal SCFAs are derivatised on the fibre in-situ with 1-pyrenyldiazomethane. With this method sharp, well-resolved chromatographic peaks were obtained with no interferences. Inclusion of deuterated analogues enabled accurate quantification. Good linearity, recoveries and precision were achieved. Differences observed between the SCFA profiles of normal subjects and patients with cystic fibrosis indicate the potential of this new technique for clinical studies. 2-Methylbutyric acid was found in all faecal samples. Few have reported this before.
G A Mills; V Walker; H Mughal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical sciences and applications     Volume:  730     ISSN:  1387-2273     ISO Abbreviation:  J. Chromatogr. B Biomed. Sci. Appl.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-09-10     Completed Date:  1999-09-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9714109     Medline TA:  J Chromatogr B Biomed Sci Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  113-22     Citation Subset:  IM    
School of Pharmacy and Biomedical Sciences, University of Portsmouth, UK.
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MeSH Terms
Case-Control Studies
Cystic Fibrosis / metabolism
Fatty Acids / analysis*
Feces / chemistry*
Indicators and Reagents / chemistry
Pyrenes / chemistry
Reproducibility of Results
Reg. No./Substance:
0/Fatty Acids; 0/Indicators and Reagents; 0/Pyrenes; 78377-23-8/1-pyrenyldiazomethane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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